+ +

•  

   

     Western blot analysis of Staufen expression in K562 cell lysate.    

  
  

•  

   

     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

•  

   

     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

Staufen antibodies are essential tools in studying the Staufen family of RNA-binding proteins, first identified in *Drosophila* for their role in mRNA localization during oogenesis and embryonic patterning. In mammals, two paralogs exist: Staufen1 (STAU1) and Staufen2 (STAU2). These proteins contain double-stranded RNA-binding domains (dsRBDs) and participate in various post-transcriptional regulatory processes, including mRNA transport, stability, translation, and decay. Staufen proteins are particularly crucial in neurons, where they regulate dendritic mRNA trafficking and local protein synthesis, impacting synaptic plasticity and long-term memory formation.

Staufen1 is implicated in Staufen-mediated decay (SMD), a pathway that degrades mRNAs by binding to secondary structures or overlapping with Alu elements in their 3'UTRs. Staufen2. while structurally similar, exhibits distinct functions, such as influencing microtubule dynamics and dendritic spine morphology. Dysregulation of Staufen proteins has been linked to neurological disorders (e.g., Alzheimer’s disease) and viral infections (e.g., HIV-1 replication exploits Staufen1 for viral RNA packaging).

Antibodies against Staufen proteins enable researchers to investigate their expression, localization, and interactions via techniques like Western blot, immunoprecipitation, and immunofluorescence. Specificity is critical, as cross-reactivity between Staufen1 and Staufen2 can occur due to sequence homology. These antibodies have advanced studies in neurobiology, virology, and disease mechanisms, underscoring Staufen's role in cellular RNA metabolism and its broader biomedical relevance.