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RhoA+B+C抗體,Rabbit Monoclonal RhoA+B+C Antibody
  • RhoA+B+C抗體,Rabbit Monoclonal RhoA+B+C Antibody
  • RhoA+B+C抗體,Rabbit Monoclonal RhoA+B+C Antibody
  • RhoA+B+C抗體,Rabbit Monoclonal RhoA+B+C Antibody

RhoA+B+C抗體;RhoA+B+C Antibody—艾普蒂

價(jià)格 詢價(jià)
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 上海
更新日期 2025-05-29
QQ交談 微信洽談

產(chǎn)品詳情

中文名稱:RhoA+B+C抗體英文名稱:Rabbit Monoclonal RhoA+B+C Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 7878 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Rabbit
偶聯(lián)物: 無(wú)靶點(diǎn): RhoA+B+C
2025-05-29 RhoA+B+C抗體 Rabbit Monoclonal RhoA+B+C Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 7878 year. Avoid repeated freeze / thaw cycles. 抗體

驗(yàn)證與應(yīng)用

應(yīng)用及物種
WB咨詢技術(shù) Human,Mouse,Rat
IF咨詢技術(shù) Human,Mouse,Rat
IHCIHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM1/20-1/100 Human,Mouse,Rat
Elisa咨詢技術(shù) Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesARH12, ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC;;RhoA/B/C
WB Predicted band size22 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human RhoA
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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       All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    


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       All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    


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       Western blot analysis of Rho A + B + C expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.    


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       Immunofluorescent analysis of Hela cells, using Rho A + B + C Antibody .    


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       Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rho A + B + C Antibody  .    


           

參考文獻(xiàn)

以下是關(guān)于RhoA/B/C抗體的3篇參考文獻(xiàn)及其簡(jiǎn)要摘要:

1. **文獻(xiàn)名稱**: *"Rho GTPases in cancer: Antibody-based detection and functional analysis"*

**作者**: Haga, R.B., Ridley, A.J.

**摘要**: 該文獻(xiàn)系統(tǒng)評(píng)估了針對(duì)RhoA、RhoB和RhoC的商用抗體的特異性,通過(guò)siRNA敲除實(shí)驗(yàn)驗(yàn)證其交叉反應(yīng)性,并比較了它們?cè)谌橄侔┙M織免疫組化中的染色效果,為癌癥研究中抗體選擇提供指導(dǎo)。

2. **文獻(xiàn)名稱**: *"Differential roles of RhoA, RhoB, and RhoC in cell migration and invasion"*

**作者**: Vega, F.M., et al.

**摘要**: 研究利用特異性抗體區(qū)分RhoA、RhoB和RhoC在腫瘤細(xì)胞中的亞細(xì)胞定位,發(fā)現(xiàn)RhoC在細(xì)胞偽足中富集且與侵襲性表型相關(guān),驗(yàn)證了抗體在功能研究中的可靠性。

3. **文獻(xiàn)名稱**: *"RhoB antibody validation for flow cytometry analysis in T-cell activation"*

**作者**: Müller, P.M., et al.

**摘要**: 開(kāi)發(fā)了一種基于流式細(xì)胞術(shù)的RhoB活性檢測(cè)方法,通過(guò)抗體阻斷實(shí)驗(yàn)證明其不與RhoA/C交叉反應(yīng),揭示了RhoB在T細(xì)胞活化中的獨(dú)特調(diào)控作用。

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**備注**:

1. RhoA/B/C抗體研究中,交叉反應(yīng)性是常見(jiàn)挑戰(zhàn),建議優(yōu)先選擇經(jīng)基因敲除/敲低驗(yàn)證的抗體(如Haga et al.的研究)。

2. 近年研究趨勢(shì)偏向開(kāi)發(fā)磷酸化特異性抗體(如p-RhoA Ser188)以檢測(cè)活性狀態(tài)。

       

背景信息

**Background of RhoA+B+C Antibody**

The RhoA+B+C antibody is a polyclonal or monoclonal reagent designed to recognize multiple isoforms of the Rho GTPase family—RhoA, RhoB, and RhoC—which belong to the Ras superfamily of small GTP-binding proteins. These isoforms share high sequence homology (≥85% amino acid identity) but exhibit distinct cellular functions and regulatory mechanisms. RhoA is widely studied for its role in stress fiber formation, cell adhesion, and actomyosin contractility. RhoB, localized to endosomes and the plasma membrane, influences vesicular trafficking, apoptosis, and growth factor responses. RhoC, structurally similar to RhoA, is often linked to cancer metastasis and invasive cell behavior.

The RhoA+B+C antibody targets a conserved epitope common to all three isoforms, enabling simultaneous detection in techniques like Western blotting, immunofluorescence, or immunohistochemistry. This multiplex recognition is advantageous for studying overlapping signaling pathways in processes such as cytoskeletal remodeling, cell migration, and tumor progression. However, due to the antibody’s cross-reactivity, complementary methods (e.g., isoform-specific knockdown or selective inhibitors) are often required to dissect individual Rho protein contributions.

Widely used in cancer research, immunology, and cell biology, this antibody helps elucidate Rho GTPase dynamics in disease mechanisms, particularly in contexts where isoform redundancy or compensatory signaling occurs. Its utility underscores the importance of Rho family proteins as therapeutic targets and biomarkers.

       
關(guān)鍵字: RhoA+B+C抗體;RhoA+B+C;RhoA+B+C Antibody;

公司簡(jiǎn)介

生物技術(shù)有限公司
成立日期 2024-07-02 (2年) 注冊(cè)資本 20萬(wàn)人民幣
員工人數(shù) 1-10人 年?duì)I業(yè)額 ¥ 100萬(wàn)以內(nèi)
主營(yíng)行業(yè) 抗體,植物生物技術(shù),細(xì)胞培養(yǎng),蛋白組學(xué) 經(jīng)營(yíng)模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 上海切爾齊生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注冊(cè)資本:20萬(wàn)人民幣
  • 企業(yè)類型:有限責(zé)任公司(自然人投資或控股)
  • 主營(yíng)產(chǎn)品:生物試劑
  • 公司地址:青浦
詢盤(pán)

RhoA+B+C抗體;RhoA+B+C Antibody—艾普蒂相關(guān)廠家報(bào)價(jià)

產(chǎn)品名稱 價(jià)格   公司名稱 報(bào)價(jià)日期
¥1098
VIP3年
上海滬震實(shí)業(yè)有限公司
2025-08-01
¥1280
湖北艾普蒂生物工程有限公司
2025-06-04
詢價(jià)
VIP3年
上海嘉定區(qū)澄瀏公路52號(hào)
2025-08-01
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