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     Western blot analysis using HIP1R mouse mAb against  A-431 lysate    

  
  

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     Lane 1: A-431 lysate ;  Lane 2: HIP1R immunoprecipitated from A-431 lysate by P15315 ;  Lane 3: The same as Lane 2 but use IgG isotype control antibody ;Result: P15315 can immunoprecipitate HIP1R;    

  
  

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     IHC-P analysis of kidney tissue by HIP1R antibody (P15315) IHC-P was performed using sections of the formalin-fixed paraffin-embedded kidney tissue;Result: Cells in tubules are positively stained at the cell membrane and microvillus.    

  
  

HIP1R (Huntingtin-interacting protein 1-related protein) is a cytosolic protein involved in clathrin-mediated endocytosis and membrane trafficking. It belongs to the HIP1 protein family, sharing structural homology with HIP1. including an N-terminal ANTH (AP180 N-terminal homology) domain for lipid binding and a C-terminal talin-like domain for actin interaction. HIP1R binds to clathrin, actin filaments, and the huntingtin protein (HTT), playing a role in maintaining synaptic vesicle recycling and cytoskeletal organization. Dysregulation of HIP1R has been linked to neurodegenerative disorders, particularly Huntington's disease, due to its interaction with mutant HTT, which may impair endocytosis and contribute to neuronal toxicity.

HIP1R antibodies are essential tools for studying its expression, localization, and function in cellular processes. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect HIP1R in various tissues, including brain samples. These antibodies help elucidate HIP1R's role in disease mechanisms, such as abnormal protein aggregation in neurodegeneration or altered membrane trafficking in cancer. Validated HIP1R antibodies often target specific domains (e.g., ANTH or C-terminal regions) and are tested for cross-reactivity in human, mouse, and rat models. Research using these antibodies has advanced understanding of HIP1R's dual roles in cellular homeostasis and pathology.