The ALKBH5 antibody is a crucial tool for studying the ALKBH5 protein, a member of the AlkB homolog (ALKBH) family of dioxygenases. ALKBH5 specifically catalyzes the demethylation of N6-methyladenosine (m6A), a dynamic and reversible RNA modification pivotal in post-transcriptional gene regulation. Discovered in 2011. ALKBH5 is predominantly localized in the nucleus and plays a key role in RNA metabolism, influencing processes such as mRNA splicing, stability, translation, and nuclear export. Its function in m6A erasure links it to diverse biological pathways, including stem cell differentiation, tumorigenesis, immune responses, and neurological disorders. Dysregulation of ALKBH5 has been implicated in cancers (e.g., glioblastoma, breast cancer), infertility, and metabolic diseases, highlighting its therapeutic potential.
ALKBH5 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP) to detect protein expression, localization, and interactions. High-quality antibodies are validated for specificity (e.g., via knockout controls) and cross-reactivity (e.g., human, mouse). Researchers rely on these antibodies to explore ALKBH5's role in m6A-mediated epigenetic regulation and its association with disease mechanisms. For instance, ALKBH5 inhibitors are being investigated as potential anticancer agents. As m6A research expands, ALKBH5 antibodies remain essential for unraveling RNA modification networks and their impact on cellular physiology and pathology.