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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Jurkat cell lysate, Primary antibody: P13373(ME2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes    

  
  

ME2 antibody targets malic enzyme 2 (ME2), a mitochondrial enzyme critical for cellular energy metabolism. ME2 catalyzes the oxidative decarboxylation of malate to pyruvate, coupled with NAD+ reduction to NADH, playing a key role in the tricarboxylic acid (TCA) cycle and linking glycolysis with mitochondrial respiration. This enzyme also contributes to biosynthetic pathways by generating precursors for lipid and nucleotide synthesis. Dysregulation of ME2 has been implicated in metabolic disorders, cancer progression, and oxidative stress responses. In cancer, ME2 overexpression is associated with enhanced tumor growth, metastasis, and chemoresistance, likely due to its role in maintaining redox balance and supporting rapid proliferation.

ME2 antibodies are essential tools for detecting ME2 protein levels in research applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They aid in studying ME2's tissue-specific expression, subcellular localization, and interactions in disease models. Validated antibodies help elucidate ME2's regulatory mechanisms in metabolic reprogramming (e.g., the Warburg effect) and its potential as a therapeutic target. Specificity is often confirmed using knockout cell lines or recombinant protein controls. Research using ME2 antibodies continues to explore its dual role in energy production and antioxidant defense, offering insights into targeting metabolic vulnerabilities in cancer and other diseases.