+ +

•  

   

     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P13129(AIFM2 Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

The AIFM2 (Apoptosis-Inducing Factor Mitochondrial 2) antibody is a tool used to detect and study the AIFM2 protein, a flavoprotein located in mitochondria. AIFM2. also known as AMID (Apoptosis-Inducing Factor-Homologous Mitochondrion-Associated Inducer of Death), shares structural similarities with AIFM1 but exhibits distinct functional roles. Unlike AIFM1. which primarily participates in caspase-independent apoptosis, AIFM2 is implicated in redox signaling, cellular metabolism, and programmed necrosis. It contains conserved FAD- and NADH-binding domains critical for its oxidoreductase activity.

Research using AIFM2 antibodies has revealed its dual role: under physiological conditions, it regulates oxidative stress response and mitochondrial homeostasis, while under pathological stress (e.g., DNA damage or hypoxia), it translocates to the nucleus to promote apoptosis. AIFM2 is overexpressed in certain cancers, correlating with tumor progression and chemotherapy resistance, making it a potential therapeutic target. The antibody is widely employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to analyze AIFM2 expression patterns, subcellular localization, and interaction partners. Recent studies also explore its involvement in ferroptosis, a lipid peroxidation-driven cell death mechanism. Validation of AIFM2 antibody specificity remains crucial, as cross-reactivity with AIFM1 or other flavoproteins may occur. Its applications span cancer biology, neurodegenerative diseases, and studies of mitochondrial dysfunction.