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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of pan Cadherin expression in C6 cell lysate.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:50 dilution.    

  
  

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     Immunohistochemical analysis of paraffin-embedded colon cancer, using pan Cadherin Antibody  .    

  
  

Pan-Cadherin antibodies are immunological tools designed to recognize a broad spectrum of cadherin family members, which are calcium-dependent transmembrane glycoproteins critical for cell-cell adhesion and tissue morphogenesis. Cadherins share a conserved extracellular domain with repeated cadherin motifs (EC domains) and a cytoplasmic domain that interacts with catenins to link the cytoskeleton. The term "pan" indicates these antibodies target conserved epitopes across multiple cadherin subtypes (e.g., E-, N-, P-cadherin), making them useful for detecting classical cadherins without subtype specificity.

These antibodies are widely employed in research to study cell adhesion dynamics, epithelial-mesenchymal transition (EMT), and tissue integrity in development, cancer metastasis, and wound healing. For example, pan-Cadherin antibodies can identify cadherin expression shifts during tumor progression or embryonic patterning. They are commonly used in techniques like immunohistochemistry, Western blotting, and immunofluorescence.

However, their broad reactivity may limit subtype-specific analyses, necessitating validation with isoform-specific antibodies for precise studies. Commercially available pan-Cadherin antibodies are often raised against conserved cytoplasmic regions (e.g., amino acid residues near the C-terminus) of human or murine cadherins, with cross-reactivity verified in multiple species. Researchers must optimize conditions to balance sensitivity and specificity, particularly in tissues with mixed cadherin expression. Overall, pan-Cadherin antibodies remain indispensable for exploring cadherin-mediated adhesion in diverse biological contexts.