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     Lane 1: 100 ng of Rat IgG1;  Lane 2: 100 ng of Rat IgG2a;  Lane 3: 100 ng of Rat IgG2b;Lane 4: 100 ng of Rat IgG2c;  Lane 5: 100 ng of Rat IgM;  Result: P15005 can detect Rat IgG2c by Western blotting;    

  
  

**Background of Rat IgG2c-HRP Antibody**

The Rat IgG2c-HRP antibody is a horseradish peroxidase (HRP)-conjugated secondary antibody specifically designed to detect rat immunoglobulin G2c (IgG2c) primary antibodies in immunoassays. Rat IgG2c, part of the IgG subclass family, shares structural similarities with mouse IgG2a/c and plays roles in immune responses, including complement activation and Fc receptor binding.

This antibody is typically produced in hosts like goats or rabbits by immunizing them with rat IgG2c fragments. Post-purification, it is conjugated to HRP, an enzyme that catalyzes substrate reactions (e.g., TMB or chemiluminescent agents) to generate detectable signals in techniques like ELISA, Western blotting, or immunohistochemistry.

The IgG2c subclass in rats is associated with Th1-biased immune responses, distinguishing it from other IgG subclasses (e.g., IgG1. IgG2a, IgG2b). The Rat IgG2c-HRP antibody’s specificity ensures minimal cross-reactivity with other rat IgG subtypes or immunoglobulins from unrelated species, making it ideal for experiments requiring precise detection.

Applications include autoimmune disease studies, infectious disease research, and monoclonal antibody characterization. Proper controls are recommended to validate specificity, and storage typically follows -20°C protocols to preserve enzymatic activity. Its utility lies in combining high sensitivity with the versatility of HRP-based detection systems.