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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: K562, HepG2 and Jurkat cell lysates, Primary antibody: P10865(FOXK1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 30 seconds    

  
  

The FOXK1 antibody is a tool used to study the FOXK1 protein, a member of the Forkhead box (Fox) family of transcription factors characterized by a conserved forkhead DNA-binding domain. FOXK1 plays critical roles in regulating cellular processes such as metabolism, autophagy, cell proliferation, and differentiation. It interacts with transcriptional co-repressors or co-activators to modulate target gene expression, often in response to nutrient availability or stress signals. For example, FOXK1 is implicated in mTOR signaling, where it regulates metabolic genes under nutrient-rich conditions, and in hypoxia-induced pathways. Dysregulation of FOXK1 has been linked to cancer progression, including tumor growth and metastasis, as well as metabolic disorders.

Antibodies targeting FOXK1 are essential for detecting its expression, localization, and interaction partners in experimental settings like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help elucidate FOXK1’s tissue-specific roles, its dynamic shuttling between the cytoplasm and nucleus, and its post-translational modifications. Recent studies also explore FOXK1’s involvement in epigenetic regulation and its crosstalk with pathways like Wnt/β-catenin and Hippo. Commercial FOXK1 antibodies are typically validated for specificity across human, mouse, and rat models, supporting both basic research and clinical investigations into FOXK1-associated diseases.