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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse muscle tissue, Primary antibody: P01890(THRA Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

The endothelin receptor type B (EDNRB) is a G protein-coupled receptor that binds endothelin peptides (ET-1. ET-2. ET-3), playing critical roles in vasoconstriction, cell proliferation, and neural crest cell development. EDNRB antibodies are tools designed to detect and study this receptor in research and diagnostic contexts. These antibodies are particularly valuable in investigating conditions linked to EDNRB dysfunction, such as Hirschsprung's disease (a congenital disorder caused by defective enteric nervous system development) and cancers like melanoma, where EDNRB overexpression may influence tumor progression and angiogenesis.

Structurally, EDNRB contains seven transmembrane domains typical of GPCRs. Its signaling pathways involve coupling with Gαi/o and Gαq proteins, regulating intracellular calcium levels, MAPK activation, and other downstream effects. Antibodies targeting EDNRB are used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess protein expression, localization, and interaction networks. Specificity and validation (e.g., knockout controls) are crucial, as cross-reactivity with related receptors (e.g., EDNRA) must be ruled out.

Research using EDNRB antibodies has also shed light on its dual roles in physiology and pathology, including its involvement in vascular remodeling, blood pressure regulation, and potential therapeutic targeting in hypertension or cancer. Recent studies explore EDNRB's role in the tumor microenvironment, particularly in immune evasion and stromal cell communication. Reliable EDNRB antibodies thus serve as essential reagents for unraveling its complex biological functions and clinical relevance.