**Background of MYL6B Antibody**
The MYL6B (myosin light chain 6B) antibody is a tool used to detect and study the MYL6B protein, a component of the myosin light chain family. MYL6B, also known as MLC1SB, is a regulatory subunit of myosin VI, a motor protein involved in intracellular transport, cell motility, and membrane trafficking. Unlike other myosin light chains, MYL6B is specifically associated with myosin VI, which moves toward the minus end of actin filaments—a unique directionality among myosin motors.
MYL6B plays a critical role in modulating myosin VI’s mechanoenzymatic activity, ensuring proper cargo binding and actin-based movement. It is ubiquitously expressed but shows higher activity in tissues requiring dynamic cytoskeletal remodeling, such as the brain, kidneys, and testes. Dysregulation of MYL6B or myosin VI has been linked to pathologies, including neurodegenerative diseases, hearing loss, and cancer metastasis.
The MYL6B antibody is widely utilized in research to investigate protein localization, expression levels, and functional interactions in cellular models. It is validated for techniques like Western blotting, immunofluorescence, and immunoprecipitation. Recent studies emphasize its role in autophagy, endosomal sorting, and cell invasion, highlighting MYL6B as a potential biomarker or therapeutic target in diseases involving cytoskeletal dysfunction. Commercial MYL6B antibodies are typically raised in rabbits or mice, with specificity confirmed via knockout controls.