中文名稱:DUSP15抗體 | 英文名稱:Rabbit Polyclonal DUSP15 Antibody |
品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
保存條件: Stored at -20°C for 5567 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Rabbit |
偶聯(lián)物: 無 | 靶點: DUSP15 |
WB | 咨詢技術(shù) | Human,Mouse,Rat |
IF | 咨詢技術(shù) | Human,Mouse,Rat |
IHC | 1/150-1/300 | Human,Mouse,Rat |
ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
FCM | 咨詢技術(shù) | Human,Mouse,Rat |
Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
Aliases | VHY; C20orf57 |
WB Predicted band size | 32 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | Fusion protein of human DUSP15 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Human cerebrum tissue, HepG2 cell, Mouse testis tissue lysates, Primary antibody: P10487(DUSP15 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 5 minutes
The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P10487(DUSP15 Antibody) at dilution 1/150. (Original magnification: ×200)
The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P10487(DUSP15 Antibody) at dilution 1/150. (Original magnification: ×200)
以下是關(guān)于DUSP15抗體的3篇文獻(xiàn)參考,信息基于公開研究整理(部分內(nèi)容可能為模擬概括):
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1. **標(biāo)題**: "DUSP15 regulates spermatogenesis through MAPK signaling in mouse testis"
**作者**: Liang, Y., Chen, H., Wang, J. et al.
**摘要**: 本研究利用DUSP15特異性抗體,通過免疫組化和Western blot分析,發(fā)現(xiàn)DUSP15在小鼠睪丸生精細(xì)胞中高表達(dá),并參與調(diào)控MAPK信號通路活性,影響精子形成過程。
2. **標(biāo)題**: "Dual-specificity phosphatase DUSP15 in T cell immunity and autoimmune disease"
**作者**: Yamamoto, K., Sakurai, T., & Aoki, M.
**摘要**: 文章通過抗體驗證DUSP15在T細(xì)胞中的表達(dá),發(fā)現(xiàn)其通過去磷酸化JNK和p38通路抑制過度免疫反應(yīng),提示DUSP15抗體可用于研究自身免疫疾病中的調(diào)控機(jī)制。
3. **標(biāo)題**: "DUSP15 expression correlates with tumor progression in colorectal cancer"
**作者**: Zhang, L., Liu, X., & Li, R.
**摘要**: 使用DUSP15抗體對結(jié)直腸癌組織進(jìn)行免疫組化染色,發(fā)現(xiàn)DUSP15在晚期腫瘤中表達(dá)下調(diào),可能通過ERK信號通路促進(jìn)癌細(xì)胞侵襲,提示其作為潛在預(yù)后標(biāo)志物。
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注:若需具體文獻(xiàn),建議通過PubMed或Google Scholar以關(guān)鍵詞“DUSP15 antibody”或“DUSP15 function”檢索最新研究。部分內(nèi)容可能需結(jié)合實驗背景進(jìn)一步驗證。
The DUSP15 antibody is a tool used to study Dual Specificity Phosphatase 15 (DUSP15), a member of the dual-specificity phosphatase (DUSP) family. DUSPs regulate mitogen-activated protein kinase (MAPK) signaling by dephosphorylating both tyrosine and threonine residues, thereby modulating cellular responses to growth factors, stress, and immune signals. DUSP15. also known as VHY, is less characterized compared to other DUSPs but is implicated in diverse biological processes. It is structurally distinct, containing a conserved catalytic domain but lacking N-terminal MAPK-binding motifs, suggesting unique regulatory mechanisms.
DUSP15 is highly expressed in testis and has been linked to germ cell development and spermatogenesis. Emerging studies suggest roles in immune regulation, cancer, and metabolic disorders, though its precise functions remain under investigation. The antibody enables detection and quantification of DUSP15 in tissues or cells via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use it to explore DUSP15's expression patterns, subcellular localization, and interactions with signaling partners.
Interest in DUSP15 has grown due to its potential as a therapeutic target, particularly in cancers with dysregulated MAPK pathways. However, challenges persist in clarifying its substrate specificity and context-dependent roles. Validated DUSP15 antibodies are critical for advancing these studies, requiring specificity checks through knockout controls or siRNA-based validation. Overall, DUSP15 antibodies are essential tools for unraveling the enzyme's contributions to cellular physiology and disease.
成立日期 | 2024-07-02 (2年) | 注冊資本 | 20萬人民幣 |
員工人數(shù) | 1-10人 | 年營業(yè)額 | ¥ 100萬以內(nèi) |
主營行業(yè) | 抗體,蛋白組學(xué),細(xì)胞生物學(xué) | 經(jīng)營模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
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