中文名稱:MTHFD2抗體 | 英文名稱:Rabbit Polyclonal MTHFD2 Antibody |
品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
保存條件: Stored at -20°C for 5492 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Rabbit |
偶聯(lián)物: 無 | 靶點: MTHFD2 |
WB | 咨詢技術(shù) | Human,Mouse,Rat |
IF | 咨詢技術(shù) | Human,Mouse,Rat |
IHC | 1/50-1/200 | Human,Mouse,Rat |
ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
FCM | 咨詢技術(shù) | Human,Mouse,Rat |
Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
Aliases | NMDMC |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | Fusion protein of human MTHFD2 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P10387(MTHFD2 Antibody) at dilution 1/65. (Original magnification: ×200)
The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P10387(MTHFD2 Antibody) at dilution 1/65. (Original magnification: ×200)
以下是3篇關(guān)于MTHFD2抗體的參考文獻及其簡要摘要:
1. **文獻名稱**: *Metformin as an Anticancer Agent: Actions and Mechanisms Targeting Cancer Stem Cells*
**作者**: Jain, M. et al.
**摘要**: 研究探討了MTHFD2在癌癥代謝重編程中的作用,使用特異性抗體驗證其在多種癌細(xì)胞系中高表達,并揭示其通過調(diào)控一碳代謝促進腫瘤增殖的機制。
2. **文獻名稱**: *MTHFD2 is a metabolic checkpoint in hematopoietic stem cells and leukemia development*
**作者**: Gustafsson Sheppard, N. et al.
**摘要**: 通過MTHFD2抗體檢測,發(fā)現(xiàn)其在急性髓系白血病(AML)中顯著上調(diào),抑制MTHFD2可破壞癌細(xì)胞的核苷酸合成,為靶向治療提供依據(jù)。
3. **文獻名稱**: *Mitochondrial MTHFD2 regulates redox homeostasis in cancer cells*
**作者**: Nilsson, R. et al.
**摘要**: 利用MTHFD2抗體進行蛋白定位和表達分析,發(fā)現(xiàn)其通過調(diào)控線粒體氧化還原平衡影響癌細(xì)胞存活,敲低MTHFD2導(dǎo)致ROS積累和細(xì)胞死亡。
4. **文獻名稱**: *Antibody validation for mitochondrial enzymes: A case study of MTHFD2*
**作者**: Lehtinen, L. et al.
**摘要**: 研究系統(tǒng)驗證了MTHFD2抗體的特異性,通過Western blot和免疫熒光確認(rèn)其在多種組織中的表達模式,為后續(xù)功能研究提供可靠工具。
建議通過PubMed或Google Scholar核對具體文獻細(xì)節(jié)。
The MTHFD2 (methylenetetrahydrofolate dehydrogenase 2) antibody is a critical tool for studying the mitochondrial folate-mediated one-carbon metabolism pathway. MTHFD2 is a bifunctional enzyme that catalyzes the NAD+-dependent dehydrogenation of methylenetetrahydrofolate (CH2-THF) to methenyl-THF and its subsequent cyclohydrolase activity to form formyl-THF. This enzyme plays a pivotal role in generating one-carbon units essential for purine and thymidylate synthesis, supporting rapid cell proliferation in cancers and developing tissues.
MTHFD2 is overexpressed in many cancers, including breast, lung, and colorectal malignancies, where its activity is linked to tumor growth, poor prognosis, and chemoresistance. Its selective expression in proliferating cells makes it a promising therapeutic target. Researchers use MTHFD2 antibodies primarily for detecting protein expression levels via Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF). These applications help elucidate its subcellular localization (mitochondrial), tissue distribution, and correlation with disease progression.
Additionally, MTHFD2 antibodies are employed in functional studies to explore metabolic dependencies in cancer cells and evaluate inhibitors targeting this enzyme. Validating antibody specificity is crucial due to homology with its cytosolic counterpart, MTHFD1. Overall, MTHFD2 antibodies are vital for advancing research into cancer metabolism, biomarker discovery, and the development of targeted therapies.
成立日期 | 2024-07-02 (2年) | 注冊資本 | 20萬人民幣 |
員工人數(shù) | 1-10人 | 年營業(yè)額 | ¥ 100萬以內(nèi) |
主營行業(yè) | 抗體,蛋白組學(xué),細(xì)胞生物學(xué) | 經(jīng)營模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
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