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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P09569(CRB3 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

CRB3 (Crumbs homolog 3) is a transmembrane protein belonging to the Crumbs family, which plays a critical role in establishing and maintaining cell polarity, apical-basal membrane organization, and tight junction formation in epithelial and endothelial cells. It is highly conserved across species and is one of three mammalian homologs (CRB1. CRB2. CRB3) of the Drosophila Crumbs protein. CRB3 is predominantly expressed in epithelial tissues, including the kidney, lung, and intestine, where it localizes to the apical membrane domain. Structurally, it contains a short extracellular domain, a single transmembrane region, and a cytoplasmic FERM-binding motif that interacts with scaffolding proteins like PATJ and PALS1 to regulate cell polarity signaling.

CRB3 antibodies are essential tools for studying its role in epithelial barrier function, morphogenesis, and diseases such as cancer or renal pathologies. These antibodies enable detection of CRB3 expression via techniques like Western blotting, immunofluorescence, or immunohistochemistry, often targeting specific regions like its intracellular C-terminal domain. Research using CRB3 antibodies has revealed its involvement in preventing epithelial-to-mesenchymal transition (EMT), modulating ciliogenesis, and maintaining tissue integrity. Dysregulation of CRB3 is linked to impaired cell polarity in cancers and polycystic kidney disease, underscoring its therapeutic and diagnostic potential.