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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse liver tissue lysate, Primary antibody: P09324(USP12 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P09324(USP12 Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

USP12 (Ubiquitin-specific protease 12) is a deubiquitinating enzyme (DUB) belonging to the ubiquitin-specific protease family, which regulates protein stability and function by removing ubiquitin chains from target substrates. It plays critical roles in diverse cellular processes, including DNA damage repair, cell cycle regulation, and apoptosis. USP12 forms a multiprotein complex with cofactors such as UAF1 (WDR48) and WDR20. which are essential for its enzymatic activity and substrate recognition. This enzyme has been implicated in modulating key signaling pathways, such as the Wnt/β-catenin and NF-κB pathways, by stabilizing proteins like β-catenin and TRAF6. Dysregulation of USP12 is associated with cancer progression, neurodegenerative diseases, and immune disorders, making it a potential therapeutic target.

Antibodies against USP12 are vital tools for studying its expression, localization, and interactions. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to explore USP12’s molecular mechanisms. High-quality USP12 antibodies exhibit specificity for distinct isoforms or post-translational modifications, enabling researchers to dissect its roles in health and disease. Validation via knockout controls or siRNA-mediated depletion is critical to confirm antibody reliability. USP12-targeting antibodies also hold promise for diagnostic applications, particularly in cancers where USP12 overexpression correlates with poor prognosis. Ongoing research aims to develop inhibitors targeting USP12’s catalytic activity, further underscoring the importance of well-characterized antibodies in both basic and translational studies.