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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: 293T cell lysate, Primary antibody: P09258(ACAT2 Antibody) at dilution 1/2000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 10 seconds    

  
  

ACAT2 (acyl-CoA:cholesterol acyltransferase 2) is an enzyme critical in cholesterol metabolism, primarily expressed in the liver and intestine. It catalyzes the esterification of free cholesterol into cholesteryl esters, which are then incorporated into lipoproteins like VLDL and chylomicrons for systemic transport. Unlike its isoform ACAT1. which is ubiquitously expressed and involved in cellular cholesterol homeostasis, ACAT2 is specifically linked to dietary cholesterol processing and atherogenic lipoprotein production.

ACAT2 antibodies are essential tools for studying the enzyme's expression, localization, and function in metabolic diseases. Researchers use these antibodies in techniques such as Western blotting, immunohistochemistry, and ELISA to investigate ACAT2's role in atherosclerosis, obesity, and metabolic syndrome. Elevated ACAT2 activity has been associated with increased accumulation of cholesterol esters in arterial plaques, making it a potential therapeutic target for cardiovascular diseases.

Current studies focus on developing ACAT2 inhibitors and understanding tissue-specific regulation. Antibodies against ACAT2 help validate animal models, assess drug efficacy, and explore gene expression patterns. Their specificity is crucial, as cross-reactivity with ACAT1 must be minimized. Ongoing research aims to clarify ACAT2's dual role in lipid absorption and inflammation, offering insights into personalized treatments for dyslipidemia and related disorders.