Active Sar1 GTP [30 μL] Catalog Number: CR-M26916 Gene Symbol: SAR1 Description: Anti Active Sar1 Mouse Monoclonal Antibody Background: Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER. Immunogen: Recombinant full length protein of active Sar1a. Applications: IP, IHC Recommended Dilutions: 1 μg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC Concentration: 1 mg/ml Host: mouse Format: Liquid Clonality: Monoclonal Isotype: IgG Purity: Purified from ascites Storage buffer: Preservative: no Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity: Anti active Sar1 antibody recognizes active Sar1 of vertebrates. Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles Immunoprecipitation/Western blot:
ar1 activation assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti active Sar1 monoclonal antibody (Cat. No. CR-M26916). Immunoblot was with an anti Sar1 rabbit polyclonal antibody (Cat. No. CR-M21090).Active Sar1 GTP [30 μL] Catalog Number: CR-M26916 Gene Symbol: SAR1 Description: Anti Active Sar1 Mouse Monoclonal Antibody Background: Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER. Immunogen: Recombinant full length protein of active Sar1a. Applications: IP, IHC Recommended Dilutions: 1 μg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC Concentration: 1 mg/ml Host: mouse Format: Liquid Clonality: Monoclonal Isotype: IgG Purity: Purified from ascites Storage buffer: Preservative: no Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity: Anti active Sar1 antibody recognizes active Sar1 of vertebrates. Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles Immunoprecipitation/Western blot: Sar1 activation assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti active Sar1 monoclonal antibody (Cat. No. CR-M26916). Immunoblot was with an anti Sar1 rabbit polyclonal antibody (Cat. No. CR-M21090).
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