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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P11516(NEK4 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P11516(NEK4 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

NEK4 (NIMA-related kinase 4) is a serine/threonine kinase belonging to the NEK family, which plays critical roles in cell cycle regulation, DNA damage response, and microtubule dynamics. Initially identified for its homology to the Aspergillus nidulans NIMA kinase, NEK4 is implicated in maintaining genomic stability by regulating the G2/M checkpoint transition and mitotic progression. Studies suggest its involvement in cilia formation, cellular stress responses, and interactions with key signaling pathways, including the Hippo and p53 pathways. Dysregulation of NEK4 has been linked to cancers, neurodegenerative disorders, and ciliopathies, highlighting its therapeutic potential.

NEK4 antibodies are essential tools for studying its expression, localization, and functional mechanisms. They enable detection of endogenous NEK4 protein levels in various tissues and cell lines via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Researchers use these antibodies to explore NEK4's phosphorylation events, substrate interactions (e.g., with PLK1 or CDK1), and its role in diseases. Commercial NEK4 antibodies are often validated for specificity, targeting unique epitopes in the kinase domain or regulatory regions. Challenges include distinguishing NEK4 isoforms and minimizing cross-reactivity with other NEK family members. High-quality monoclonal antibodies are increasingly developed to enhance reproducibility in diagnostic and basic research applications.