+ +

•  

   

     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Mouse brain tissue, Primary antibody: P11345(SHANK1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

SHANK1 antibodies are essential tools in neuroscience research, targeting the SHANK1 protein, a key member of the SHANK/ProSAP family of synaptic scaffolding proteins. SHANK1 is predominantly localized at the postsynaptic density (PSD) of excitatory synapses, where it organizes neurotransmitter receptors, ion channels, and signaling molecules by linking membrane proteins to the actin cytoskeleton. It plays a critical role in synaptic plasticity, neural development, and cognitive functions. Dysregulation of SHANK1 has been implicated in neurodevelopmental and psychiatric disorders, including autism spectrum disorders (ASD) and schizophrenia.

SHANK1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to detect protein expression, localization, and interactions. These antibodies typically recognize specific domains (e.g., ANK repeats, SH3. PDZ, or proline-rich regions) or epitopes of SHANK1. with variations in clonal specificity (monoclonal vs. polyclonal) influencing experimental outcomes. Researchers often validate SHANK1 antibodies using knockout models or knockdown controls to ensure specificity, as cross-reactivity with other SHANK family members (SHANK2. SHANK3) can occur due to structural homology.

Studies leveraging SHANK1 antibodies have advanced understanding of synaptic pathology, protein networks in neurological diseases, and potential therapeutic targets. However, discrepancies in antibody performance across studies highlight the need for rigorous validation and standardized protocols.