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ETFA抗體,Mouse Monoclonal ETFA Antibody
  • ETFA抗體,Mouse Monoclonal ETFA Antibody
  • ETFA抗體,Mouse Monoclonal ETFA Antibody
  • ETFA抗體,Mouse Monoclonal ETFA Antibody

ETFA抗體;ETFA Antibody—艾普蒂

價(jià)格 詢(xún)價(jià)
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 上海
更新日期 2025-05-25
QQ交談 微信洽談

產(chǎn)品詳情

中文名稱(chēng):ETFA抗體英文名稱(chēng):Mouse Monoclonal ETFA Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 1449 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類(lèi)別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Mouse
偶聯(lián)物: 無(wú)靶點(diǎn): ETFA
2025-05-25 ETFA抗體 Mouse Monoclonal ETFA Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 1449 year. Avoid repeated freeze / thaw cycles. 抗體

驗(yàn)證與應(yīng)用

應(yīng)用及物種
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨詢(xún)技術(shù) Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesEMA; GA2; MADD
Entrez GeneID2108
clone2F8A5
WB Predicted band size35kDa
Host/IsotypeMouse IgG2a
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human ETFA (AA: 134-333) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using ETFA mouse mAb against .HepG2 (1), A431 (2), Hek293 (3),Hela (4)and MCF-7 (5) cell lysate.    


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       Immunofluorescence analysis of Hela cells using ETFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hela cells using ETFA mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of HepG2 cells using ETFA mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using ETFA mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using ETFA mouse mAb with DAB staining.    


           

參考文獻(xiàn)

以下是3篇關(guān)于ETFA抗體的參考文獻(xiàn)示例(注:以下內(nèi)容為模擬虛構(gòu),僅作參考格式示例):

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1. **文獻(xiàn)名稱(chēng)**: "Deficiency of Electron Transfer Flavoprotein Alpha (ETFA) Identified via Immunoblotting in a Patient with Glutaric Acidemia Type II"

**作者**: Smith, J.R., et al.

**摘要**: 本研究通過(guò)Western Blot技術(shù)結(jié)合特異性ETFA抗體,在一名Ⅱ型戊二酸血癥患者中發(fā)現(xiàn)ETFA蛋白表達(dá)缺失,揭示了ETFA基因突變與線粒體脂肪酸氧化障礙的關(guān)聯(lián)。

2. **文獻(xiàn)名稱(chēng)**: "Localization of ETFA in Mouse Skeletal Muscle Using Immunofluorescence: Implications for Metabolic Myopathies"

**作者**: Tanaka, M., & Yamamoto, S.

**摘要**: 利用ETFA抗體進(jìn)行免疫熒光染色,發(fā)現(xiàn)ETFA蛋白在小鼠骨骼肌線粒體中的特異性定位,為研究代謝性肌病中線粒體功能異常提供了形態(tài)學(xué)依據(jù)。

3. **文獻(xiàn)名稱(chēng)**: "Development and Validation of a Novel ETFA Monoclonal Antibody for Diagnostic Applications"

**作者**: Chen, L., et al.

**摘要**: 文章報(bào)道了一種高親和力ETFA單克隆抗體的開(kāi)發(fā),并通過(guò)ELISA和免疫組化驗(yàn)證其在診斷遺傳性代謝疾病中的靈敏度和特異性,為臨床檢測(cè)提供新工具。

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*提示*:如需真實(shí)文獻(xiàn),建議通過(guò)PubMed或Google Scholar檢索關(guān)鍵詞“ETFA antibody”、“Electron Transfer Flavoprotein Alpha”+“antibody application”。實(shí)際研究中,ETFA抗體常用于代謝疾病診斷(如多種?;o酶A脫氫酶缺乏癥,MADD)及線粒體功能研究。

       

背景信息

The ETFA antibody targets the alpha subunit of the Electron Transfer Flavoprotein (ETF), a critical component in mitochondrial energy metabolism. ETF, composed of alpha (ETFA) and beta (ETFB) subunits, acts as an electron carrier in fatty acid β-oxidation and amino acid catabolism. It shuttles electrons from acyl-CoA dehydrogenases to the respiratory chain via ETF-ubiquinone oxidoreductase (ETF-QO). ETFA mutations are linked to genetic disorders like glutaric acidemia type II (GA-II), a rare autosomal recessive condition characterized by metabolic acidosis, hypoglycemia, and cardiomyopathy.

ETFA antibodies are essential tools in research to study ETF expression, localization, and function in cellular models or tissues. They are used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to assess protein levels in metabolic studies or disease models. Researchers also employ these antibodies to investigate the molecular mechanisms underlying GA-II and other mitochondrial disorders, aiding in diagnostic approaches or therapeutic development.

Commercial ETFA antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Specific validation steps (e.g., knockout controls) are crucial to ensure antibody reliability. Understanding ETFA's role and its antibody applications enhances insights into mitochondrial dysfunction and potential treatments for metabolic diseases.

       
關(guān)鍵字: ETFA抗體;ETFA;ETFA Antibody;

公司簡(jiǎn)介

生物技術(shù)有限公司
成立日期 2024-07-02 (2年) 注冊(cè)資本 20萬(wàn)人民幣
員工人數(shù) 1-10人 年?duì)I業(yè)額 ¥ 100萬(wàn)以?xún)?nèi)
主營(yíng)行業(yè) 抗體,植物生物技術(shù),細(xì)胞培養(yǎng),蛋白組學(xué) 經(jīng)營(yíng)模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 上海切爾齊生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注冊(cè)資本:20萬(wàn)人民幣
  • 企業(yè)類(lèi)型:有限責(zé)任公司(自然人投資或控股)
  • 主營(yíng)產(chǎn)品:生物試劑
  • 公司地址:青浦
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