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NDC80抗體,Mouse Monoclonal NDC80 Antibody
  • NDC80抗體,Mouse Monoclonal NDC80 Antibody
  • NDC80抗體,Mouse Monoclonal NDC80 Antibody
  • NDC80抗體,Mouse Monoclonal NDC80 Antibody

NDC80抗體;NDC80 Antibody—艾普蒂

價(jià)格 詢(xún)價(jià)
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 上海
更新日期 2025-05-25
QQ交談 微信洽談

產(chǎn)品詳情

中文名稱(chēng):NDC80抗體英文名稱(chēng):Mouse Monoclonal NDC80 Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 1448 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類(lèi)別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Mouse
偶聯(lián)物: 無(wú)靶點(diǎn): NDC80
2025-05-25 NDC80抗體 Mouse Monoclonal NDC80 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 1448 year. Avoid repeated freeze / thaw cycles. 抗體

驗(yàn)證與應(yīng)用

應(yīng)用及物種
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨詢(xún)技術(shù) Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC技術(shù)咨詢(xún) Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesHEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
Entrez GeneID10403
clone4B12H3
WB Predicted band size74kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human NDC80 (AA: 443-642) expressed in Mammalian.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using NDC80 mouse mAb against Jurkat (1) cell lysate.    


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       Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of HepG2 cells using NDC80 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of Jurkat cells using NDC80 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of K562 cells using NDC80 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using NDC80 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using NDC80 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using NDC80 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using NDC80 mouse mAb with DAB staining.    


           

參考文獻(xiàn)

以下是關(guān)于NDC80抗體的3-4篇參考文獻(xiàn)示例(文獻(xiàn)信息為模擬,僅供參考):

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1. **文獻(xiàn)名稱(chēng)**:*"The Ndc80 complex bridges microtubule-kinetochore interactions in mitosis"*

**作者**:Cheeseman, I.M. et al.

**摘要**:本研究利用NDC80抗體進(jìn)行免疫沉淀和功能分析,闡明了NDC80復(fù)合體在染色體分離中連接微管與動(dòng)粒的關(guān)鍵作用,并揭示其缺失導(dǎo)致染色體排列異常。

2. **文獻(xiàn)名稱(chēng)**:*"Structure of the Ndc80 kinetochore complex and its role in microtubule binding"*

**作者**:Ciferri, C. et al.

**摘要**:通過(guò)冷凍電鏡和抗體標(biāo)記技術(shù),解析了NDC80復(fù)合體的三維結(jié)構(gòu),證明其通過(guò)卷曲螺旋結(jié)構(gòu)域與微管結(jié)合,為有絲分裂中動(dòng)粒組裝提供結(jié)構(gòu)基礎(chǔ)。

3. **文獻(xiàn)名稱(chēng)**:*"NDC80 phosphorylation regulates kinetochore-microtubule attachments and mitotic progression"*

**作者**:DeLuca, J.G. et al.

**摘要**:利用磷酸化特異性NDC80抗體,發(fā)現(xiàn)Aurora B激酶介導(dǎo)的NDC80磷酸化調(diào)控動(dòng)粒-微管附著的穩(wěn)定性,確保染色體正確分離。

4. **文獻(xiàn)名稱(chēng)**:*"NDC80 overexpression as a prognostic biomarker in breast cancer"*

**作者**:Wang, Y. et al.

**摘要**:通過(guò)免疫組化(NDC80抗體)分析乳腺癌組織,發(fā)現(xiàn)NDC80高表達(dá)與患者生存率低相關(guān),提示其作為潛在腫瘤標(biāo)志物的價(jià)值。

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注:以上文獻(xiàn)信息為示例,實(shí)際引用時(shí)請(qǐng)核實(shí)真實(shí)文獻(xiàn)的準(zhǔn)確性。建議通過(guò)PubMed或Google Scholar搜索關(guān)鍵詞“NDC80 antibody”或“NDC80 function”獲取最新研究。

       

背景信息

The NDC80 antibody is a crucial tool for studying the NDC80 complex, a conserved heterotetrameric protein assembly (comprising NDC80/HEC1. NUF2. SPC24. and SPC25) essential for proper chromosome segregation during mitosis. This complex forms part of the KMN network (KNL1. MIS12. NDC80) at kinetochores, mediating stable microtubule attachment and ensuring accurate chromosome alignment on the mitotic spindle. NDC80 antibodies are widely used to investigate mitotic mechanisms, checkpoint signaling, and errors linked to chromosomal instability—a hallmark of cancer and developmental disorders.

Researchers employ these antibodies in techniques like immunofluorescence, Western blotting, and immunoprecipitation to visualize NDC80 localization, quantify expression levels, or study protein-protein interactions. Specific epitopes targeted by commercial antibodies (e.g., recognizing human NDC80/HEC1’s N-terminal region) help dissect structural-functional relationships. Studies using NDC80 antibodies have revealed its role in correcting microtubule-kinetochore misattachments and maintaining tension across sister chromatids. Dysregulation of NDC80 is implicated in tumorigenesis, making it a potential biomarker or therapeutic target. However, antibody validation remains critical, as off-target binding can complicate interpretations in diverse experimental models.

       
關(guān)鍵字: NDC80抗體;NDC80;NDC80 Antibody;

公司簡(jiǎn)介

生物技術(shù)有限公司
成立日期 2024-07-02 (2年) 注冊(cè)資本 20萬(wàn)人民幣
員工人數(shù) 1-10人 年?duì)I業(yè)額 ¥ 100萬(wàn)以?xún)?nèi)
主營(yíng)行業(yè) 抗體,植物生物技術(shù),細(xì)胞培養(yǎng),蛋白組學(xué) 經(jīng)營(yíng)模式 貿(mào)易,工廠(chǎng),試劑,定制,服務(wù)
  • 上海切爾齊生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注冊(cè)資本:20萬(wàn)人民幣
  • 企業(yè)類(lèi)型:有限責(zé)任公司(自然人投資或控股)
  • 主營(yíng)產(chǎn)品:生物試劑
  • 公司地址:青浦
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