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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)    

  
  

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     Immunofluorescence analysis of Hela cells using TIA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Hela cells using TIA1 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using TIA1 mouse mAb with DAB staining.    

  
  

TIA1 (T-cell intracellular antigen 1) antibody is a tool used to detect the presence of TIA1 protein, a RNA-binding protein involved in regulating gene expression and cellular stress responses. TIA1. primarily located in cytoplasmic stress granules, plays a role in mRNA splicing, translation repression, and promoting apoptosis under stress conditions. Structurally, it contains RNA recognition motifs (RRMs) and a prion-like domain, enabling interactions with nucleic acids and proteins.

In diagnostic pathology, TIA1 antibody is utilized as an immunohistochemical marker to identify cytotoxic T lymphocytes and natural killer (NK) cells, aiding in the classification of lymphoproliferative disorders. For example, it helps distinguish aggressive cytotoxic T-cell lymphomas from other subtypes. Additionally, TIA1 expression is studied in autoimmune diseases (e.g., myositis) and neurodegenerative conditions, where aberrant stress granule dynamics may contribute to pathogenesis.

Research applications focus on TIA1's dual role in tumorigenesis—acting as a tumor suppressor by promoting apoptosis or as an oncogenic factor in certain contexts. Mutations in TIA1 are linked to rare disorders like Welander distal myopathy. The antibody's specificity and relevance in stress-response pathways make it valuable for both clinical diagnostics and molecular studies exploring RNA metabolism, cellular stress, and immune-mediated diseases.