The PPP1R13L (Protein Phosphatase 1 Regulatory Subunit 13 Like) gene, also known as RAI or ASPP1. encodes a regulatory protein involved in modulating cellular stress responses and apoptosis. It interacts with key signaling pathways, including the p53 tumor suppressor network, by binding to proteins like p53 and NF-κB to influence their transcriptional activity. PPP1R13L is implicated in regulating cell survival decisions under stress conditions, such as DNA damage or oxidative stress, making it a focus in cancer and neurodegenerative disease research.
Antibodies targeting PPP1R13L are essential tools for studying its expression, localization, and function. These antibodies are typically raised in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They enable detection of PPP1R13L via techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Validation includes verifying specificity through knockout cell lines or siRNA knockdowns to ensure minimal cross-reactivity with homologous proteins like ASPP2.
Research applications of PPP1R13L antibodies span investigating its role in apoptosis regulation, cancer progression (e.g., as a potential biomarker), and neurological disorders. Dysregulation of PPP1R13L has been linked to altered p53 activity in tumors and neuroinflammation pathways. Commercially available antibodies vary in clonality (monoclonal/polyclonal) and epitope recognition, necessitating careful selection based on experimental needs. Recent studies also explore its involvement in viral infection responses, highlighting its broader therapeutic relevance.