The NUDT2 antibody is a crucial tool for studying the NUDT2 protein, a member of the Nudix hydrolase family. NUDT2. also known as diadenosine tetraphosphate hydrolase, catalyzes the hydrolysis of dinucleotide polyphosphates like Ap4A, regulating their cellular levels. These metabolites play roles in stress response, DNA repair, and RNA processing. The antibody enables detection and localization of NUDT2 in various experimental settings, including Western blotting, immunohistochemistry, and immunofluorescence.
Research highlights NUDT2's involvement in multiple biological processes. It modulates mRNA capping by interacting with the cytoplasmic capping complex, influencing gene expression. Dysregulation of NUDT2 has been linked to cancer progression, neurodevelopmental disorders, and viral infection responses. For instance, reduced NUDT2 expression correlates with poor prognosis in certain cancers, while its overexpression may affect neuronal differentiation.
Commercially available NUDT2 antibodies are typically raised against specific epitopes, often in rabbit or mouse hosts. Validation includes testing reactivity across species (human, mouse, rat) and confirming absence of cross-reactivity with other Nudix family members. Challenges remain in ensuring antibody specificity due to conserved domains within the Nudix family. Recent studies utilizing NUDT2 antibodies have advanced understanding of its non-canonical roles, such as regulating tRNA fragmentation and cellular redox homeostasis, underscoring its therapeutic potential.