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     Gel: 8%SDS-PAGE, Lysate: 60 μg, Lane 1-2: HT29 cells, hela cells, Primary antibody: P08681(RBBP5 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

The RBBP5 (Retinoblastoma-Binding Protein 5) antibody is a critical tool for studying epigenetic regulation and transcriptional control. RBBP5 is a conserved nuclear protein that plays a scaffolding role in multi-subunit histone methyltransferase complexes, including the SET1/MLL family complexes responsible for catalyzing histone H3 lysine 4 (H3K4) methylation—a hallmark of active gene promoters. By interacting with proteins like MLL1/KMT2A and ASH2L, RBBP5 stabilizes these complexes and enhances their enzymatic activity, influencing processes such as embryonic development, hematopoiesis, and cellular differentiation. Dysregulation of RBBP5 is linked to cancers, developmental disorders, and other diseases.

Antibodies targeting RBBP5 are widely used in research to investigate its expression, localization, and molecular interactions. They enable techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and co-immunoprecipitation (Co-IP), helping dissect its role in chromatin remodeling and gene regulation. Validated RBBP5 antibodies typically show specificity in detecting the ~60 kDa protein across human, mouse, and rat samples, often confirmed via knockout (KO) controls. These reagents are essential for exploring RBBP5's involvement in disease mechanisms or its crosstalk with signaling pathways, supporting advances in epigenetics and therapeutic discovery.