RACK1 (Receptor for Activated C Kinase 1) is a highly conserved scaffolding protein belonging to the WD40 repeat family, playing a pivotal role in diverse cellular processes. It was initially identified as an anchoring protein for protein kinase C (PKC), facilitating its translocation and activation. Beyond PKC signaling, RACK1 interacts with multiple partners, including Src kinases, integrins, growth factor receptors, and ribosomal components, regulating pathways tied to cell growth, migration, apoptosis, and immune responses. Its involvement in ribosome binding also links it to translational control.
RACK1 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to explore RACK1’s role in physiological and pathological contexts. Dysregulation of RACK1 has been implicated in cancers, neurodegenerative diseases, and immune disorders, making its antibodies valuable for mechanistic and diagnostic research.
Commercially available RACK1 antibodies are typically raised against conserved regions (e.g., human residues 165-317), with validation across species (human, mouse, rat). Clonality (monoclonal/polyclonal) and host species (rabbit, mouse) vary, tailored to experimental needs. Proper controls (e.g., knockout cell lines) are critical due to potential cross-reactivity with other WD40 proteins. Overall, RACK1 antibodies serve as key reagents in unraveling its multifaceted roles in cellular signaling and disease pathogenesis.