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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: HT29 cells, Hela cells, 293T cells, Primary antibody: P08583(NUSAP1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

  
  

The NUSAP1 (Nucleolar and Spindle Associated Protein 1) antibody is a tool used to detect and study the NUSAP1 protein, a critical regulator of mitotic progression and chromosomal stability. NUSAP1 is a microtubule-associated protein enriched during the G2/M phase of the cell cycle, playing roles in spindle assembly, chromosome segregation, and cytokinesis. It contains distinct domains for microtubule binding, chromatin interaction, and protein recruitment, enabling its involvement in maintaining genomic integrity. Dysregulation of NUSAP1 has been linked to cancer progression, with overexpression observed in malignancies like breast, prostate, and colorectal cancers, where it correlates with poor prognosis, metastasis, and therapy resistance.

The NUSAP1 antibody is widely utilized in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression levels, subcellular localization, and functional interactions in both research and clinical contexts. Studies employing this antibody have highlighted NUSAP1's potential as a biomarker for cancer diagnosis and as a therapeutic target, given its role in promoting cell proliferation and survival. Additionally, NUSAP1 inhibition has been shown to induce mitotic defects and apoptosis in cancer cells, underscoring its relevance in oncology research. The antibody's specificity and reliability make it essential for elucidating NUSAP1's mechanisms in cell cycle regulation and cancer biology.