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     The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using P08564(NEDD4L Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

NEDD4L (Neural precursor cell Expressed Developmentally Down-regulated 4-Like) is a HECT-type E3 ubiquitin ligase involved in regulating protein turnover via the ubiquitin-proteasome system. It plays critical roles in cellular processes such as ion channel regulation, membrane protein trafficking, and signaling pathway modulation. Structurally, NEDD4L contains a C2 domain (mediating membrane binding), multiple WW domains (substrate recognition), and a catalytic HECT domain. Its activity is tightly controlled through phosphorylation (e.g., by SGK1) and alternative splicing, particularly in regulatory regions affecting substrate specificity.

NEDD4L antibodies are essential tools for studying its expression patterns, subcellular localization, and interactions with substrates like ENaC (epithelial sodium channel) and SMAD2/3. These antibodies enable detection through techniques including Western blotting, immunohistochemistry, and immunoprecipitation. Research applications span hypertension studies (via ENaC regulation in kidneys), cancer biology (modulating PTEN/TP53 pathways), and neurological disorders (synaptic protein regulation). Validated antibodies help distinguish between NEDD4L isoforms generated by alternative promoters and splicing events. As NEDD4L dysfunction is implicated in Liddle syndrome, pulmonary hypertension, and tumor progression, specific antibodies support both mechanistic studies and therapeutic development targeting its ubiquitination activity. Proper antibody validation via knockout controls remains crucial given sequence similarities within the NEDD4 ubiquitin ligase family.