The RPS27 antibody is a research tool designed to detect and analyze the Ribosomal Protein S27 (RPS27), a component of the 40S ribosomal subunit involved in protein synthesis. RPS27. also known as Metallopanstimulin-1 (MPS-1), plays dual roles in cellular processes. Beyond its canonical function in ribosome assembly and translation, RPS27 has been implicated in DNA repair, redox regulation, and cell proliferation, with studies linking its overexpression to cancers such as hepatocellular carcinoma, breast cancer, and leukemia. This has spurred interest in RPS27 as a potential biomarker or therapeutic target.
RPS27 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to study protein expression, localization, and interactions. Polyclonal and monoclonal variants are available, often validated for specificity using knockout or knockdown controls. Researchers employ these antibodies to explore RPS27's role in tumorigenesis, drug resistance, and cellular stress responses. Recent studies also investigate its involvement in non-canonical pathways, including p53 modulation and ubiquitin-like post-translational modifications.
Commercial RPS27 antibodies are typically raised against epitopes in human or mouse RPS27. with cross-reactivity varying by species. Proper validation remains critical due to occasional off-target binding. Overall, RPS27 antibodies serve as essential tools for unraveling the multifaceted biology of this ribosomal protein in health and disease.