"HL-60人原髓細胞白血病復(fù)蘇細胞保種中心|帶STR證書

傳代比例：1:2-1:4(首次傳代建議1:2)

生長特性：懸浮生長

細胞凍存復(fù)蘇材料與方法步驟：常用的細胞冷凍貯存器為貯存器，規(guī)格有３５L和５０L兩種。使用時要注意以下幾點：(１)一般兩周需充一次，至少一個月充一次。溫度達-１９６℃，使用時注意勿讓濺到皮膚上，以免引起凍傷。(２)容器為雙層結(jié)構(gòu)，中間為真空層，瓶口有雙層焊接處，應(yīng)防止焊接部裂開。(３)在裝入時，要注意緩慢小心，并用厚紙卷筒或制漏斗作引導(dǎo)，使直達瓶底，如有專用灌注裝置則更HAO。若為初次使用，加時更要緩慢，以免溫度驟降而使容器損壞。細胞凍存時常備的材料有：０.２５%胰蛋白酶，含１０%～２０%的血清培養(yǎng)，DMSO(分析純)或無色新鮮甘油(１２１°C蒸氣GAO壓消毒)，２mL安瓿(或?qū)Ｓ眉毎麅龃婀?、吸管、離心管、噴燈、紗布袋(或凍存管架)等。主要操作步驟為：(１)選擇處于對數(shù)生長期的細胞，在凍存前一天ZuiHAO換。將多個培養(yǎng)瓶中的細胞培養(yǎng) 去掉，用０.２５%胰蛋白酶消化。適時去掉胰蛋白酶，加入少量新培養(yǎng)。用吸管吸取培養(yǎng)反復(fù)吹打瓶壁上的細胞，使其成為均勻分散的細胞懸。懸浮生產(chǎn)細胞則不要消化處理。然后將細胞收集于離心管中離心(１０００r/min，１０分鐘)。(２)去上清，加入含２０%小牛血清的完全培養(yǎng)基，于４℃預(yù)冷１５分鐘后，逐滴加入已無菌的DMSO或甘油，用吸管輕輕吹打使細胞均勻，細胞濃度為３×１０６～１×１０７/mL之間。(３)將上述細胞分裝于安瓿或?qū)Ｓ美鋬鏊芰瞎苤?，安瓿裝１～１.５mL在火焰噴燈上封口，封口處要完全封閉，圓滑無勾。冷凍管要將蓋子蓋緊，并標記HAO細胞名稱和凍存日期，同時作HAO登記(日期、細胞種類及代次、凍存支數(shù))。(４)將裝HAO細胞的安瓿或凍存管裝入沙布袋內(nèi);置于容器頸口處存放過夜，次日轉(zhuǎn)入中。采用控制降溫速度的方法也可采用下列步驟：先將安瓿置入４℃冰箱中２～３小時，再移至冰箱冷凍室內(nèi)３～４小時，再吊入容器頸氣態(tài)部分存放２小時，Zui后沉入中。細胞凍存在中可以長期保存，但為妥善起見，凍存半年后，ZuiHAO取出一只安瓿細胞復(fù)蘇培養(yǎng)，觀察生長情況，然后再繼續(xù)凍存。

換液周期：每周2-3次

J82COT Cells;背景說明：電子顯微鏡下未觀察到橋粒但觀察到數(shù)目不同的粗面內(nèi)質(zhì)網(wǎng)和突出微絲。 含ras (H-ras)癌基因。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮細胞樣;相關(guān)產(chǎn)品有：SK MES 1細胞、P3-X63-Ag 8.653細胞、SJCRH30細胞

Epstein-Barr-3 Cells;背景說明：該細胞源自一名３歲患有Burkitt＇s淋巴瘤的黑人男孩的B淋巴細胞，EBNA陽性。;傳代方法：１：２-１：４傳代，每周２-３次。;生長特性：懸浮生長;形態(tài)特性：淋巴母細胞樣;相關(guān)產(chǎn)品有：SHG44細胞、Roswell Park Memorial Institute 2650細胞、NEC-8細胞

HEK 293-F Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代;生長特性：貼壁生長；懸浮生長;形態(tài)特性：上皮細胞樣;相關(guān)產(chǎn)品有：TR 146細胞、Chang liver細胞、RPMI No. 1846細胞

HL-60人原髓細胞白血病復(fù)蘇細胞保種中心|帶STR證書

背景信息：該細胞由CollinsSJ從一位患有急性早幼粒細胞性白血病的３６歲白人女性的外周血中分離建立；可自發(fā)分化，或在鹽、次黃嘌呤、佛波醇肉豆蔻(,TPA)、DMSO(１%to１.５%)、D和視黃的刺激下發(fā)生分化；刺激后可分泌TNF-α。該細胞具有吞噬活性和趨化反應(yīng)，癌基因myc陽性，表達補體受體和FcR。

細胞系的選擇需要考慮到細胞系的功能特點、生長速率、鋪板效率、生長條件和生長特征、克隆效率、培養(yǎng)方式等因素，如果您想高產(chǎn)量表達重組蛋白，您可以選擇可以懸浮生長的快速生長細胞系。細胞培養(yǎng)的操作步驟主要包括傳代、換液、凍存和復(fù)蘇。這些步驟確保了細胞能夠在實驗室環(huán)境中長期存活并繼續(xù)增殖。傳代是將細胞從一個容器轉(zhuǎn)移到另一個容器的過程，以擴大細胞數(shù)量；換液是為了清除代謝廢物并補充新鮮培養(yǎng)基；凍存則是為了長期保存細胞，而復(fù)蘇則是重新激活冷凍保存的細胞使其恢復(fù)正常生長。

產(chǎn)品包裝：復(fù)蘇發(fā)貨：T25培養(yǎng)瓶(一瓶)或凍存發(fā)貨：1ml凍存管(兩支)

來源說明：細胞主要來源ATCC、ECACC、DSMZ、RIKEN等細胞庫

KYSE-510 Cells;背景說明：食管鱗癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：OVCAR432細胞、KE37細胞、B78細胞

MDCKII-WT Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：３傳代，３-４天傳１次;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：MMAc-SF細胞、HRGEC細胞、JVM2細胞

H-2195 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H-226細胞、Hs683細胞、D324 Med細胞

Fu97 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：KRC/Y細胞、T 84細胞、LN 229細胞

HL-60人原髓細胞白血病復(fù)蘇細胞保種中心|帶STR證書

物種來源：人源、鼠源等其它物種來源

形態(tài)特性：淋巴母細胞樣

我常看到一個比較復(fù)雜的四步消化法，這個挺有意思，我也是第一次聽說，應(yīng)該是網(wǎng)友自己發(fā)展出來的方法，很有參考價值。但我估計這個方法可能對付少數(shù)非常怪異的細胞才需要這么復(fù)雜的程序。我目前養(yǎng)過的近３００株細胞里面，還沒遇到這么怪異的。比如Caco２其實是很HAO消化的細胞，不需要胰酶孵育即可，只是有點成片分布。不要以為成片分布是自己沒養(yǎng)HAO，這個視細胞而定。如果和標準形態(tài)不一致，那可能是自己沒消化HAO導(dǎo)致的，但如果消化方法正確，仍然成片分布，甚至完全吹打成單細胞懸，貼壁后仍然成片分布，這是細胞的性，是因為貼壁過程中重新聚集了。這個時候你拼了命要去讓它均勻分布，你的細胞之后會對你越來越不HAO；比較難消化的細胞(潤洗方法５min還不能消化)，就以colon canr為例，比如HC１５, LS４１１和KM１２，這樣的細胞一般需要用少量胰酶孵育，但也不需要很多，一般１００ mm dish，一次Zui多加入５００ul，就足夠了，一般我加３００ul。即使這樣難消化的細胞，一般不超過５min，即可見細胞成片移動，就應(yīng)該停止消化。一些正常細胞也會有難消化的時候，比如sDC細胞，但也只要用少量胰酶孵育，３min左右即可看到成片沙狀移動。

JM-Jurkat Cells;背景說明：該細胞源自一位１４歲患有T淋巴細胞白血病男性的外周血;傳代方法：保持細胞密度在３—９×１０５cells/ml之間，１：５—１：１０傳代，每周換液２—３次;生長特性：懸浮生長;形態(tài)特性：圓形，單個或呈片;相關(guān)產(chǎn)品有：PFSK-1細胞、TE85細胞、Hs274T細胞

PC14 Cells;背景說明：肺腺癌；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：LMTK-細胞、Sup T-1細胞、HOPC細胞

AN-3 Cells;背景說明：AN３CA細胞建系于１９６４年。它衍生于子宮內(nèi)膜癌患者淋巴結(jié)轉(zhuǎn)移組織，具有癌細胞的基本特性，能在體外長期傳代培養(yǎng)，接種實驗動物產(chǎn)生明顯腫瘤。但細胞的生物學(xué)特性及超微結(jié)構(gòu)尚未深入研究，僅發(fā)現(xiàn)該細胞系促黑激素合成為陰性。細胞常用于人子宮內(nèi)膜癌細胞生物學(xué)及其相關(guān)特性研究。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：U373MG細胞、COLO 320細胞、HCM細胞

hFOB1.19 Cells;背景說明：成骨細胞；SV４０轉(zhuǎn)化；條件永生；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Helacyton gartleri細胞、IPLB-SF 21細胞、Ramos細胞

CAL 85-1 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Intestinal Epithelioid Cell line No. 18細胞、HIBEpiC細胞、SCC-7細胞

SHEP1 Cells;背景說明：神經(jīng)母細胞瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SKRC-39細胞、Panc-08.13細胞、Hs940T細胞

AMJ2-C8 Cells;背景說明：巨噬細胞；J２病毒轉(zhuǎn)化；C５７BL/６J;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：ANA1細胞、CHO K1細胞、SGC 7901細胞

RPMI8402 Cells;背景說明：急性T淋巴細胞白血病；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：C-33 A細胞、MDCC MSB1細胞、Rat 1細胞

UCLA-SO-M21 Cells;背景說明：黑色素瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NK92MI細胞、STO細胞、MV-3細胞

Y3-Ag1,2,3 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HO-8910細胞、CD18/HPAF細胞、LLC-MK2細胞

EB3 [Human Burkitt lymphoma] Cells;背景說明：該細胞源自一名３歲患有Burkitt＇s淋巴瘤的黑人男孩的B淋巴細胞，EBNA陽性。;傳代方法：１：２-１：４傳代，每周２-３次。;生長特性：懸浮生長;形態(tài)特性：淋巴母細胞樣;相關(guān)產(chǎn)品有：Okayama University Medical School-27細胞、H929細胞、SW480E細胞

SF-539 Cells;背景說明：膠質(zhì)瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SCCVII細胞、HCC4006細胞、UMR 106細胞

FRO81-2 Cells;背景說明：未分化甲狀腺癌；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：RIN-m細胞、SW1088細胞、NCI-H196細胞

TE4 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：RKO_AS45細胞、SNB-19細胞、BrCL18細胞

NS-1-Ag4-1 Cells;背景說明：這是P３X６３Ag８(ATCCTIB-９)的一個不分泌克隆。Kappa鏈合成了但不分泌。能抗０.１mM８-氮雜鳥嘌呤但不能在HAT培養(yǎng)基中生長。據(jù)報道它是由于缺失了３-酮類固醇還原酶活性的膽固醇營養(yǎng)缺陷型。檢測表明肢骨發(fā)育畸形病毒(鼠痘)陰性。;傳代方法：１：２傳代，３天內(nèi)可長滿。;生長特性：懸浮生長;形態(tài)特性：淋巴母細胞;相關(guān)產(chǎn)品有：H661細胞、Tb1Lu細胞、OUMS27細胞

Hs 888.T Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代；每周換液２-３次;生長特性：貼壁生長;形態(tài)特性：成纖維細胞和上皮細胞的混合樣;相關(guān)產(chǎn)品有：SPDC-CCL141細胞、LC-MS細胞、TCC-PAN2細胞

Tu-212 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代;生長特性：貼壁生長　;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SNU-475細胞、NCI-H526細胞、V79-4細胞

HL-60人原髓細胞白血病復(fù)蘇細胞保種中心|帶STR證書

Abcam HEK293T NME1 KO Cells(提供STR鑒定圖譜)

AG11426 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line RRA003 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line XE051 Cells(提供STR鑒定圖譜)

BY00118 Cells(提供STR鑒定圖譜)

CRICKi008-A Cells(提供STR鑒定圖譜)

DA04291 Cells(提供STR鑒定圖譜)

Endeavour-2 Cells(提供STR鑒定圖譜)

GM06000 Cells(提供STR鑒定圖譜)

MRCV Cells;背景說明：MRC-５細胞系來自１４周齡男性胎兒的正常肺組織，該細胞老化前能傳代４２~４６個倍增時間。;傳代方法：１：２-１：５傳代；每周１-２次。;生長特性：貼壁生長;形態(tài)特性：成纖維細胞樣;相關(guān)產(chǎn)品有：NCI-H524細胞、BEP2D細胞、D-283細胞

MM1S Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：４傳代，２-３天換液１次。;生長特性：混合生長;形態(tài)特性：淋巴母細胞樣;相關(guān)產(chǎn)品有：L-1210細胞、FAT細胞、B5537SKIN細胞

X63-Ag 8.6.5.3 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MX1細胞、FRH-0201細胞、H-1581細胞

CNLMG-B5537SKIN Cells;背景說明：成纖維 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：UPCI:SCC90細胞、L 540細胞、SK-N-BE2細胞

WM266 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：KE-39細胞、NTERA2-cloneD1細胞、293EBNA細胞

MLOY4 Cells;背景說明：骨；SV４０轉(zhuǎn)化;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：PC615.3細胞、GM346細胞、GM00346細胞

Abgenix 4.48.3 Cells(提供STR鑒定圖譜)

OACP4 C Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H1581細胞、LLCPK1細胞、MOLT4細胞

LAD2 Cells;背景說明：肥大 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：IPEC-1細胞、RGB細胞、WERIRb1細胞

RPMI8226/S Cells;背景說明：來源于一位６１歲的男性漿細胞瘤患者；可產(chǎn)生免疫球蛋白輕鏈，未檢測到重鏈。;傳代方法：按１：２傳代，５-６小時可以看到細胞分裂;生長特性：懸浮生長;形態(tài)特性：淋巴母細胞樣;相關(guān)產(chǎn)品有：HFL-1細胞、SMA-560細胞、BEL7404細胞

AtT 20 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HN13細胞、A549/ATCC細胞、MCF7/WT細胞

P3-NS1/1-Ag4-1 Cells;背景說明：這是P３X６３Ag８(ATCCTIB-９)的一個不分泌克隆。Kappa鏈合成了但不分泌。能抗０.１mM８-氮雜鳥嘌呤但不能在HAT培養(yǎng)基中生長。據(jù)報道它是由于缺失了３-酮類固醇還原酶活性的膽固醇營養(yǎng)缺陷型。檢測表明肢骨發(fā)育畸形病毒(鼠痘)陰性。;傳代方法：１：２傳代，３天內(nèi)可長滿。;生長特性：懸浮生長;形態(tài)特性：淋巴母細胞;相關(guān)產(chǎn)品有：NHLF細胞、NCI-H524細胞、Caco 2細胞

Tn-5B1-4 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：OSC-19細胞、MNNG-HOS細胞、HL-60 Clone 15細胞

Kit225 K6 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：2780細胞、PA12細胞、NCI-H508細胞

NP69 Cells;背景說明：鼻咽；上皮細胞；SV４０轉(zhuǎn)化;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H-2023細胞、LM(TK-)細胞、NCI-H1573細胞

GM21098 Cells(提供STR鑒定圖譜)

HAP1 LMNA (-) 3 Cells(提供STR鑒定圖譜)

CCD-18Co Cells;背景說明：結(jié)腸；成纖維細胞；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：TW 01細胞、NCI-157細胞、A2780 CP70細胞

SKLU-1 Cells;背景說明：該細胞系源于一位６０歲的白人女性患者的肺腺癌組織。;傳代方法：１：２傳代；每周換液２次。;生長特性：貼壁生長;形態(tài)特性：上皮細胞樣;相關(guān)產(chǎn)品有：SK-Mel1細胞、WEHI 3細胞、D283-MED細胞

MDA-415 Cells;背景說明：這株細胞表達WNT７B癌基因。８１６８０８８].帶瘤患者來自巴拉圭，雖然填報的是白人，但細胞表型存在G６PDＡ型，顯示其屬于混血。細胞株形成平展延伸的上皮細胞樣，在電鏡下呈現(xiàn)結(jié)節(jié)，伴隨著延伸的微管和微板。不容易用胰酶消化。;傳代方法：消化５-１０分鐘。１：２。４-５天長滿。;生長特性：貼壁生長;形態(tài)特性：上皮細胞;相關(guān)產(chǎn)品有：HUVEC細胞、Mouse INsulinoma 6細胞、C22 (Clara)細胞

NL20 Cells;背景說明：支氣管上皮細胞；SV４０轉(zhuǎn)化；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：RIN m5F細胞、MLFC細胞、LTEP-sm 1細胞

H-2405 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：５-１：８傳代;生長特性：混合生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Lu-99A細胞、WiDr細胞、WM115F細胞

TFH Cells;背景說明：輔助性T Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：RAW 264.7細胞、NU-GC-2細胞、OCILY-10細胞

MC3T3, E1 subgroup-4 clone Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Ketr-3細胞、HAPI細胞、C-6細胞

MDA-MB-231-luc Cells;背景說明：MDA-MB-２３１來自患有轉(zhuǎn)移乳腺腺癌的５１歲女病人的胸水。在裸鼠和ALS處理的BALB/c小鼠中，它能形成低分化腺癌（III級）。;傳代方法：１：２傳代;生長特性：貼壁生長　;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：211H細胞、Co320細胞、HFF1細胞

HPSI0313i-cesj_1 Cells(提供STR鑒定圖譜)

K562 eGFP-TEAD2 Cells(提供STR鑒定圖譜)

MEF (DR4) IRR Cells(提供STR鑒定圖譜)

NH50178 Cells(提供STR鑒定圖譜)

QIMR-DAR Cells(提供STR鑒定圖譜)

Tp301MG Cells(提供STR鑒定圖譜)

UiPSC-005 Cells(提供STR鑒定圖譜)

HF7450-Cas9 Cells(提供STR鑒定圖譜)

P3X63 Ag8 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H-2291細胞、FCCH1024細胞、SU-DHL6細胞

Chinese Hamster Lung Cells;背景說明：廣泛應(yīng)用于染色體異常測試。;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：上皮細胞樣;相關(guān)產(chǎn)品有：H-1568細胞、PNT1-a細胞、COLO-320-DM細胞

R-1059-D Cells;背景說明：骨肉瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：DanG細胞、C41細胞、RBL-1細胞

SUM 159PT Cells;背景說明：乳腺癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：GM02132細胞、TE-5細胞、P3-X63Ag8.653細胞

High 5 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：PANC 327細胞、MA104細胞、MKN7細胞

High 5 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：PANC 327細胞、MA104細胞、MKN7細胞

KU.812 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代。３天內(nèi)可長滿;生長特性：懸浮生長;形態(tài)特性：骨髓母細胞;相關(guān)產(chǎn)品有：J-774A.1細胞、Hi-five細胞、HEK-293-H細胞

BNL.1ME A.7R.1 Cells;背景說明：肝；上皮細胞；BALB/c;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCI-H740細胞、NCI-SNU-C1細胞、3T3-Swiss albino細胞

MDCK Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代;生長特性：貼壁生長　;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SK-BR-3細胞、PNEC30細胞、293/EBNA-1細胞

HL-60 clone15 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：維持細胞濃度在１×１０５-１×１０６/ml,每２-３天換液１次。;生長特性：懸浮生長;形態(tài)特性：淋巴母細胞樣;相關(guān)產(chǎn)品有：MLOY4細胞、Tn-5B1-4細胞、NIH-OVCAR-3細胞

HFSF Cells;背景說明：北京眼科研究所建系;傳代方法：１：３傳代，３-４天換液一次;生長特性：貼壁生長;形態(tài)特性：成纖維細胞樣;相關(guān)產(chǎn)品有：526-mel細胞、C4-I細胞、TE15細胞

HIEC Cells;背景說明：腸；上皮 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Hs 600.T細胞、DoHH-2細胞、CCRF.CEM細胞

NCIH378 Cells;背景說明：小細胞肺癌；胸腔積液轉(zhuǎn)移；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：COLO320-DM細胞、COS-7細胞、EC9706細胞

NL20SV Cells;背景說明：支氣管上皮細胞；SV４０轉(zhuǎn)化；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MA-c細胞、NCI-SNU-398細胞、Jiyoye細胞

NCI-H2347 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：６傳代,每周２-３次。;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：KATO III細胞、32D/cl3細胞、KAT-5細胞

SPPHIi005-A Cells(提供STR鑒定圖譜)

HPAFII Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：UM-UC-1細胞、SKG-3a細胞、Centre Antoine Lacassagne-85-1細胞

HFLS-OA Cells;背景說明：滑膜；關(guān)節(jié)炎成纖維 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HFF-1細胞、L5178-R細胞、COV 434細胞

RBL-I Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：PFSK1細胞、B16細胞、Institute for Medical Research-32細胞

HRA-19 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCI-H441細胞、aTC1-6細胞、HL 60細胞

MDCK-2 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：３傳代，３-４天傳１次;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：Ku812F細胞、HS578T細胞、Swiss/3T3細胞

Transformed C3H Mouse Kidney-1 Cells;背景說明：腎小管；上皮細胞；SV４０轉(zhuǎn)化；C３H/Mai;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SW-1088細胞、NK92-MI細胞、HAEC細胞

Factor Dependent Cell-Paterson 1 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：２-３天換液１次;生長特性：懸浮生長;形態(tài)特性：淋巴母細胞;相關(guān)產(chǎn)品有：HeLa S-3細胞、RPMI-8402細胞、G-Olig2細胞

MDAMB157 Cells;背景說明：該細胞源自一位患有乳腺髓樣癌的４４歲黑人女性，表達WNT７B癌基因，細胞與細胞邊界處有細胞橋粒、微絨毛、張力細絲。;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：MRC-V細胞、H-596細胞、no-11細胞

BT 20 Cells;背景說明：該細胞１９５８年由E.Y. Lasfargues 和 L. Ozzello 建系，源自一位７４歲白人女性的乳腺癌組織。該細胞表達WNT３和WNT７８。TNF alpha抑制該細胞生長。該細胞雌激素受體陰性，但表達５＇外顯子缺失的雌激素mRNA。;傳代方法：１：２—１：４傳代，２—３天換液一次;生長特性：貼壁生長;形態(tài)特性：上皮細胞樣;相關(guān)產(chǎn)品有：NCI-HUT-460細胞、NE-4C細胞、NCI-H2122細胞

NCIH508 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：UACC893細胞、NCI-H2228細胞、MOLT3細胞

RSC-364 Cells;背景說明：滑膜 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCI-H-128細胞、CV-1細胞、F442-A細胞

SK Mel 2 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：３-１：６傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：多邊形的;相關(guān)產(chǎn)品有：VK2細胞、SKMEL-2細胞、JB6 clone 30, subclone 7b細胞

NG 108-15 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Hepa1-6細胞、CL1細胞、Tadarida brasiliensis 1 lung細胞

HBL-1 [Human diffuse large B-cell lymphoma] Cells;背景說明：彌漫大B淋巴瘤；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：EFM192細胞、HTh 74細胞、MC57細胞

HL-60人原髓細胞白血病復(fù)蘇細胞保種中心|帶STR證書

BayGenomics ES cell line RRO340 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line YHB404 Cells(提供STR鑒定圖譜)

HB1.1-EI Cells(提供STR鑒定圖譜)

PCRP-BACH2-3B8 Cells(提供STR鑒定圖譜)

BC35 Cells(提供STR鑒定圖譜)

HPS0186 Cells(提供STR鑒定圖譜)

" "PubMed=6255959; DOI=10.1016/0014-4827(81)90422-5

Olsson I., Olofsson T.

Induction of differentiation in a human promyelocytic leukemic cell line (HL-60). Production of granule proteins.

Exp. Cell Res. 131:225-230(1981)

PubMed=6945469; DOI=10.1016/0145-2126(81)90018-7

Major P.P., Griffin J.D., Minden M.D., Kufe D.W.

A blast subclone of the HL-60 human promyelocytic cell line.

Leuk. Res. 5:429-430(1981)

PubMed=6954533; DOI=10.1073/pnas.79.7.2194; PMCID=PMC346157

Westin E.H., Gallo R.C., Arya S.K., Eva A., Souza L.M., Baluda M.A., Aaronson S.A., Wong-Staal F.

Differential expression of the amv gene in human hematopoietic cells.

Proc. Natl. Acad. Sci. U.S.A. 79:2194-2198(1982)

PubMed=6091813; DOI=10.1182/blood.V64.5.1059.1059

Palumbo A., Minowada J., Erikson J., Croce C.M., Rovera G.

Lineage infidelity of a human myelogenous leukemia cell line.

Blood 64:1059-1063(1984)

PubMed=6582512; DOI=10.1073/pnas.81.2.568; PMCID=PMC344720

Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.

Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.

Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=2858093; DOI=10.1073/pnas.82.3.790; PMCID=PMC397132

Wolf D., Rotter V.

Major deletions in the gene encoding the p53 tumor antigen cause lack of p53 expression in HL-60 cells.

Proc. Natl. Acad. Sci. U.S.A. 82:790-794(1985)

PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0

Drexler H.G., Gaedicke G., Minowada J.

Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 carboxylic esterase.

Leuk. Res. 9:209-229(1985)

PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1

Drexler H.G., Gaedicke G., Minowada J.

Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C. 3.2.1.30).

Leuk. Res. 9:549-559(1985)

PubMed=3860286

Bhalla K.N., Hindenburg A.A., Taub R.N., Grant S.

Isolation and characterization of an anthracycline-resistant human leukemic cell line.

Cancer Res. 45:3657-3662(1985)

PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x

Drexler H.G., Gaedicke G., Minowada J.

Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.

Leuk. Res. 9:537-548(1985)

PubMed=3311197; DOI=10.1182/blood.V70.5.1233.1233

Collins S.J.

The HL-60 promyelocytic leukemia cell line: proliferation, differentiation, and cellular oncogene expression.

Blood 70:1233-1244(1987)

DOI=10.1159/000415060

Morikawa S., Harada T., Katoh T.

Heterogeneity of cellular origins in human malignant lymphoma cell line derived from histo-monocytic lineage cells.

(In book chapter) Cellular, molecular, genetic approaches to immunodiagnosis and immunotherapy. 8th International conference on labeled antibodies, Tokyo, November 1985; Kano K., Mori S., Sugisaki T., Torisu M. (eds.); pp.373-380; Karger; Basel; Switzerland (1988)

PubMed=3335022

Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Cancer Res. 48:589-601(1988)

PubMed=3422031; DOI=10.1182/blood.V71.1.242.242

Dalton W.T. Jr., Ahearn M.J., McCredie K.B., Freireich E.J., Stass S.A., Trujillo J.M.

HL-60 cell line was derived from a patient with FAB-M2 and not FAB-M3.

Blood 71:242-247(1988)

PubMed=2545346

Hindenburg A.A., Gervasoni J.E. Jr., Krishna S., Stewart V.J., Rosado M., Lutzky J., Bhalla K.N., Baker M.A., Taub R.N.

Intracellular distribution and pharmacokinetics of daunorubicin in anthracycline-sensitive and -resistant HL-60 cells.

Cancer Res. 49:4607-4614(1989)

PubMed=1970118; DOI=10.1128/mcb.10.5.2154-2163.1990; PMCID=PMC360563

Collins S.J., Robertson K.A., Mueller L.

Retinoic acid-induced granulocytic differentiation of HL-60 myeloid leukemia cells is mediated directly through the retinoic acid receptor (RAR-alpha).

Mol. Cell. Biol. 10:2154-2163(1990)

PubMed=2140233; DOI=10.1111/j.1440-1827.1990.tb01549.x

Nakano A., Harada T., Morikawa S., Kato Y.

Expression of leukocyte common antigen (CD45) on various human leukemia/lymphoma cell lines.

Acta Pathol. Jpn. 40:107-115(1990)

PubMed=1368402; DOI=10.1007/BF02540028

Schumpp-Vonach B., Schlaeger E.-J.

Growth study of lactate and ammonia double-resistant clones of HL-60 cells.

Cytotechnology 8:39-44(1992)

PubMed=1574572; DOI=10.2307/3578273

Dunphy E.J., Beckett M.A., Thompson L.H., Weichselbaum R.R.

Expression of the polymorphic human DNA repair gene XRCC1 does not correlate with radiosensitivity in the cells of human head and neck tumor cell lines.

Radiat. Res. 130:166-170(1992)

PubMed=8316623; DOI=10.2307/3578190

Evans H.H., Ricanati M., Horng M.-F., Jiang Q.-Y., Mencl J., Olive P.L.

DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines.

Radiat. Res. 134:307-315(1993)

PubMed=8343448

Kajigaya Y., Sasaki H., Ikuta K., Matsuyama S., Hirabayashi Y., Inoue T.

Serum-free culture for leukemia cells.

Hum. Cell 6:49-56(1993)

PubMed=7630190

Zhou M.-X., Gu L.-B., James C.D., He J., Yeager A.M., Smith S.D., Findley H.W. Jr.

Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia.

Leukemia 9:1159-1161(1995)

PubMed=8562479

Banerjee D., Lenz H.-J., Schnieders B., Manno D.J., Ju J.-F., Spears C.P., Hochhauser D., Danenberg K.D., Danenberg P.V., Bertino J.R.

Transfection of wild-type but not mutant p53 induces early monocytic differentiation in HL60 cells and increases their sensitivity to stress.

Cell Growth Differ. 6:1405-1413(1995)

PubMed=8558913

Morita S., Tsuchiya S., Fujie H., Itano M., Ohashi Y., Minegishi M., Imaizumi M., Endo M., Takano N., Konno T.

Cell surface c-kit receptors in human leukemia cell lines and pediatric leukemia: selective preservation of c-kit expression on megakaryoblastic cell lines during adaptation to in vitro culture.

Leukemia 10:102-105(1996)

PubMed=9434637; DOI=10.1006/excr.1997.3813

Piepmeier E.H., Kalns J.E., McIntyre K.M., Lewis M.L.

Prolonged weightlessness affects promyelocytic multidrug resistance.

Exp. Cell Res. 237:410-418(1997)

PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x; PMCID=PMC5921588

Hosoya N., Hangaishi A., Ogawa S., Miyagawa K., Mitani K., Yazaki Y., Hirai H.

Frameshift mutations of the hMSH6 gene in human leukemia cell lines.

Jpn. J. Cancer Res. 89:33-39(1998)

PubMed=9557624; DOI=10.1038/sj.leu.2400976

Mattii L., Barale R., Petrini M.

Use of the comet test in the evaluation of multidrug resistance of human cell lines.

Leukemia 12:627-632(1998)

PubMed=9607592

Ju J.-F., Banerjee D., Lenz H.-J., Danenberg K.D., Schmittgen T.D., Spears C.P., Schonthal A.H., Manno D.J., Hochhauser D., Bertino J.R., Danenberg P.V.

Restoration of wild-type p53 activity in p53-null HL-60 cells confers multidrug sensitivity.

Clin. Cancer Res. 4:1315-1322(1998)

PubMed=9737686; DOI=10.1038/sj.leu.2401112

Zhang W.-J., Ohnishi K., Shigeno K., Fujisawa S., Naito K., Nakamura S., Takeshita K., Takeshita A., Ohno R.

The induction of apoptosis and cell cycle arrest by arsenic trioxide in lymphoid neoplasms.

Leukemia 12:1383-1391(1998)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x; PMCID=PMC5921886

Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.

Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.

Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=10739008; DOI=10.1016/S0145-2126(99)00182-4

Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.

Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.

Leuk. Res. 24:255-262(2000)

DOI=10.1016/B978-0-12-221970-2.50457-5

Drexler H.G.

The leukemia-lymphoma cell line factsbook.

(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=11226526; DOI=10.1016/S0145-2126(00)00121-1

Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.

Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000), 255-262.

Leuk. Res. 25:275-278(2001)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459

Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.

Short tandem repeat profiling provides an international reference standard for human cell lines.

Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=12068308; DOI=10.1038/nature00766

Davies H.R., Bignell G.R., Cox C., Stephens P.J., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C.S., Shipley J.M., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H.F., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.

Mutations of the BRAF gene in human cancer.

Nature 417:949-954(2002)

PubMed=12661003; DOI=10.1002/gcc.10196

Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.F., Schlag P.M., Scherneck S.

Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.

Genes Chromosomes Cancer 37:29-35(2003)

PubMed=16120335; DOI=10.1016/S1567-7249(03)00010-2

Levin B.C., Holland K.A., Hancock D.K., Coble M., Parsons T.J., Kienker L.J., Williams D.W., Jones M., Richie K.L.

Comparison of the complete mtDNA genome sequences of human cell lines -- HL-60 and GM10742A -- from individuals with pro-myelocytic leukemia and Leber hereditary optic neuropathy, respectively, and the inclusion of HL-60 in the NIST human mitochondrial DNA standard reference material -- SRM 2392-I.

Mitochondrion 2:387-400(2003)

PubMed=15843827; DOI=10.1038/sj.leu.2403749

Andersson A., Eden P., Lindgren D., Nilsson J., Lassen C., Heldrup J., Fontes M., Borg A., Mitelman F., Johansson B., Hoglund M., Fioretos T.

Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.

Leukemia 19:1042-1050(2005)

PubMed=16408098; DOI=10.1038/sj.leu.2404081

Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.

JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.

Leukemia 20:471-476(2006)

PubMed=17088437; DOI=10.1158/1535-7163.MCT-06-0433; PMCID=PMC2705832

Ikediobi O.N., Davies H.R., Bignell G.R., Edkins S., Stevens C., O'Meara S., Santarius T., Avis T., Barthorpe S., Brackenbury L., Buck G., Butler A.P., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Hunter C., Jenkinson A., Jones D., Kosmidou V., Lugg R., Menzies A., Miroo T., Parker A., Perry J., Raine K.M., Richardson D., Shepherd R., Small A., Smith R., Solomon H., Stephens P.J., Teague J.W., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Reinhold W.C., Weinstein J.N., Stratton M.R., Futreal P.A., Wooster R.

Mutation analysis of 24 known cancer genes in the NCI-60 cell line set.

Mol. Cancer Ther. 5:2606-2612(2006)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

PubMed=22068913; DOI=10.1073/pnas.1111840108; PMCID=PMC3219108

Gillet J.-P., Calcagno A.M., Varma S., Marino M., Green L.J., Vora M.I., Patel C., Orina J.N., Eliseeva T.A., Singal V., Padmanabhan R., Davidson B., Ganapathi R., Sood A.K., Rueda B.R., Ambudkar S.V., Gottesman M.M.

Redefining the relevance of established cancer cell lines to the study of mechanisms of clinical anti-cancer drug resistance.

Proc. Natl. Acad. Sci. U.S.A. 108:18708-18713(2011)

PubMed=22032829; DOI=10.1016/j.mrfmmm.2011.10.005

Luan Y., Kogi M., Rajaguru P., Ren J., Yamaguchi T., Suzuki K., Suzuki T.

Microarray analysis of responsible genes in increased growth rate in the subline of HL60 (HL60RG) cells.

Mutat. Res. 731:20-26(2012)

PubMed=22347499; DOI=10.1371/journal.pone.0031628; PMCID=PMC3276511

Ruan X.-Y., Kocher J.-P.A., Pommier Y., Liu H.-F., Reinhold W.C.

Mass homozygotes accumulation in the NCI-60 cancer cell lines as compared to HapMap trios, and relation to fragile site location.

PLoS ONE 7:E31628-E31628(2012)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027

Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Nature 483:603-607(2012)

PubMed=22944676; DOI=10.1515/hsz-2012-0195

Reinke S.O., Bayer M., Berger M., Hinderlich S., Blanchard V.

The analysis of N-glycans of cell membrane proteins from human hematopoietic cell lines reveals distinctions in their pattern.

Biol. Chem. 393:731-747(2012)

PubMed=23955599; DOI=10.1038/ng.2731

Kon A., Shih L.-Y., Minamino M., Sanada M., Shiraishi Y., Nagata Y., Yoshida K.-i., Okuno Y., Bando M., Nakato R., Ishikawa S., Sato-Otsubo A., Nagae G., Nishimoto A., Haferlach C., Nowak D., Sato Y., Alpermann T., Nagasaki M., Shimamura T., Tanaka H., Chiba K., Yamamoto R., Yamaguchi T., Otsu M., Obara N., Sakata-Yanagimoto M., Nakamaki T., Ishiyama K., Nolte F., Hofmann W.-K., Miyawaki S., Chiba S., Mori H., Nakauchi H., Koeffler H.P., Aburatani H., Haferlach T., Shirahige K., Miyano S., Ogawa S.

Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.

Nat. Genet. 45:1232-1237(2013)

PubMed=24368162; DOI=10.1016/j.exphem.2013.12.004

Sripayap P., Nagai T., Uesawa M., Kobayashi H., Tsukahara T., Ohmine K., Muroi K., Ozawa K.

Mechanisms of resistance to azacitidine in human leukemia cell lines.

Exp. Hematol. 42:294-306.e2(2014)

PubMed=24670534; DOI=10.1371/journal.pone.0092047; PMCID=PMC3966786

Varma S., Pommier Y., Sunshine M., Weinstein J.N., Reinhold W.C.

High resolution copy number variation data in the NCI-60 cancer cell lines from whole genome microarrays accessible through CellMiner.

PLoS ONE 9:E92047-E92047(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652

Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080

Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.

A comprehensive transcriptional portrait of human cancer cell lines.

Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397

Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.

A resource for cell line authentication, annotation and quality control.

Nature 520:307-311(2015)

PubMed=25894527; DOI=10.1371/journal.pone.0121314; PMCID=PMC4404347

Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A.S., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.

A mass spectrometric-derived cell surface protein atlas.

PLoS ONE 10:E0121314-E0121314(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878

Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Miroo T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

PubMed=28109323; DOI=10.1186/s13045-017-0396-0; PMCID=PMC5251306

Masetti R., Bertuccio S.N., Astolfi A., Chiarini F., Lonetti A., Indio V., De Luca M., Bandini J., Serravalle S., Franzoni M., Pigazzi M., Martelli A.M., Basso G., Locatelli F., Pession A.

Hh/Gli antagonist in acute myeloid leukemia with CBFA2T3-GLIS2 fusion gene.

J. Hematol. Oncol. 10:26.1-26.5(2017)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076

Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.

Characterization of human cancer cell lines by reverse-phase protein arrays.

Cancer Cell 31:225-239(2017)

PubMed=29491412; DOI=10.1038/s41388-018-0150-2; PMCID=PMC5955861

Gerlach D., Tontsch-Grunt U., Baum A., Popow J., Scharn D., Hofmann M.H., Engelhardt H., Kaya O., Beck J., Schweifer N., Gerstberger T., Zuber J., Savarese F., Kraut N.

The novel BET bromodomain inhibitor BI 894999 represses super-enhancer-associated transcription and synergizes with CDK9 inhibition in AML.

Oncogene 37:2687-2701(2018)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5; PMCID=PMC6167786

Tan K.-T., Ding L.-W., Sun Q.-Y., Lao Z.-T., Chien W., Ren X., Xiao J.-F., Loh X.-Y., Xu L., Lill M., Mayakonda A., Lin D.-C., Yang H.H., Koeffler H.P.

Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.

BMC Cancer 18:940.1-940.13(2018)

PubMed=30629668; DOI=10.1371/journal.pone.0210404; PMCID=PMC6328144

Uphoff C.C., Pommerenke C., Denkmann S.A., Drexler H.G.

Screening human cell lines for viral infections applying RNA-Seq data analysis.

PLoS ONE 14:E0210404-E0210404(2019)

PubMed=30670178; DOI=10.1016/j.jcf.2018.06.007

Jennings S., Ng H.P., Wang G.-S.

Establishment of a DeltaF508-CF promyelocytic cell line for cystic fibrosis research and drug screening.

J. Cyst. Fibros. 18:44-53(2019)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675

Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)"