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     Immunohistochemical analysis of paraffin-embedded human brain glioma tissue showing nuclear localization using calcyclin mouse mAb with DAB staining.    

  
  

The ONECUT1 antibody is a crucial tool for studying the ONECUT1 transcription factor, also known as Hepatocyte Nuclear Factor 6 (HNF6). ONECUT1 belongs to a family of DNA-binding proteins characterized by a single cut domain and a homeodomain, enabling sequence-specific gene regulation. It plays pivotal roles in organ development, particularly in the liver, pancreas, and nervous system. During embryogenesis, ONECUT1 regulates hepatocyte differentiation, pancreatic beta-cell maturation, and neurogenesis. In adults, it maintains metabolic homeostasis and tissue-specific gene expression. Dysregulation of ONECUT1 is linked to diseases like diabetes, liver fibrosis, and hepatocellular carcinoma.

Researchers use ONECUT1 antibodies primarily in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression, localization, and dynamics in cells and tissues. These antibodies aid in exploring developmental pathways, disease mechanisms, and regenerative processes. For example, studies employ ONECUT1 antibodies to investigate beta-cell dysfunction in diabetes or liver regeneration post-injury. Commercially available antibodies are often raised in rabbits or mice, validated for specificity via knockout controls. Their application spans basic research, drug discovery, and translational studies, underscoring their importance in understanding ONECUT1’s dual roles in development and disease.