The ARHGAP45 (Rho GTPase Activating Protein 45) antibody is a tool used to study the function and expression of the ARHGAP45 protein, a member of the RhoGAP family. ARHGAP45 regulates Rho GTPases, including RhoA, Rac1. and Cdc42. by enhancing their GTPase activity, thereby influencing cytoskeletal dynamics, cell migration, and immune cell signaling. It is highly expressed in hematopoietic cells and plays a role in modulating T-cell activation, neutrophil migration, and platelet function. Dysregulation of ARHGAP45 has been linked to immune disorders, leukemia, and inflammatory diseases.
ARHGAP45 antibodies are designed to detect specific isoforms or post-translational modifications of the protein in applications such as Western blotting, immunofluorescence, and flow cytometry. These antibodies aid in elucidating ARHGAP45's role in pathways like PI3K/AKT and its interaction with signaling molecules such as VAV1. Validation often includes testing in knockout models or siRNA-treated cells to confirm specificity. Researchers use these antibodies to explore ARHGAP45's involvement in pathological processes, including cancer metastasis and autoimmune conditions, making them valuable for both mechanistic studies and potential therapeutic targeting. Commercial ARHGAP45 antibodies are typically raised in rabbits or mice, with reactivity across human, mouse, and rat samples.