CLPX antibody targets the CLPX protein, a critical component of the mitochondrial ATP-dependent Clp protease system. CLPX, a member of the AAA+ ATPase family, regulates the Clp protease complex (ClpXP) by facilitating substrate recognition, unfolding, and translocation into the proteolytic ClpP subunit. This system is essential for maintaining mitochondrial protein homeostasis, degrading misfolded or damaged proteins, and regulating specific substrates involved in metabolism, stress responses, and organelle dynamics. Dysregulation of CLPX is linked to mitochondrial dysfunction, neurodegenerative diseases, and cancer.
CLPX antibodies are widely used in research to study protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. They enable investigations into CLPX's role in mitochondrial quality control, its regulatory mechanisms under stress (e.g., oxidative damage), and its partnership with ClpP. These antibodies are typically generated using recombinant CLPX protein fragments as immunogens in animal hosts (e.g., rabbits, mice), followed by affinity purification. Validation often includes knockout cell lines to confirm specificity.
As mitochondrial proteostasis gains attention in disease research, CLPX antibodies serve as vital tools for exploring therapeutic targets and mechanistic pathways linked to aging, metabolic disorders, and cancer progression.