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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: K562 and Hela cell lysates, Primary antibody: P05927(PICK1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

The PICK1 (Protein Interacting with C Kinase 1) antibody is a crucial tool for studying the role of the PICK1 protein in cellular processes. PICK1 is a scaffolding protein containing a PDZ domain and a lipid-binding BAR domain, enabling interactions with multiple partners such as protein kinase Cα (PKCα), AMPA receptors, and dopamine transporters. It regulates synaptic plasticity, receptor trafficking, and neurotransmitter release, particularly in the central nervous system. Dysregulation of PICK1 is linked to neurological disorders, including epilepsy, neuropathic pain, and psychiatric conditions like schizophrenia.

Antibodies targeting PICK1 are widely used in neuroscience and cell biology research to detect its expression, localization, and interaction networks. They facilitate techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation, aiding in the exploration of PICK1’s involvement in synaptic function and disease mechanisms. For example, studies using PICK1 antibodies have revealed its role in AMPA receptor internalization during long-term depression, a key process in learning and memory. Additionally, these antibodies help assess PICK1’s potential as a therapeutic target, with some research focusing on inhibiting its interactions to modulate neuropathic pain or addiction-related behaviors. Commercial PICK1 antibodies are typically raised in rabbits or mice, validated for specificity across human, rat, and mouse models.