The PDS5A antibody is a crucial tool in studying the PDS5A protein, a key component of the cohesin complex involved in chromosome organization and sister chromatid cohesion during cell division. Cohesin, composed of SMC1A, SMC3. RAD21. and regulatory subunits like PDS5A, ensures accurate chromosome segregation, DNA repair, and transcriptional regulation. PDS5A (also known as AFG3L1P or hPDS5) interacts with cohesin to modulate its dynamics, influencing chromatin loop formation and gene expression. Unlike its paralog PDS5B, PDS5A exhibits distinct tissue-specific expression patterns and roles in developmental processes.
Antibodies targeting PDS5A enable researchers to investigate its expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These studies are vital for understanding cohesin-related disorders, including Cornelia de Lange syndrome and cancers linked to cohesin mutations. Dysregulation of PDS5A has been implicated in genomic instability and tumorigenesis, making it a potential biomarker or therapeutic target.
Commercial PDS5A antibodies are typically validated for specificity across human and model organisms, though cross-reactivity with PDS5B remains a consideration. Recent research also explores PDS5A's non-canonical roles in DNA replication and stem cell differentiation. Its antibody thus serves as a gateway to unraveling cohesin's multifaceted functions in health and disease.