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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: PC-3 cell lysate, Primary antibody: P02841(MED28 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 10 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P02841(MED28 Antibody) at dilution 1/240. (Original magnification: ×200)    

  
  

MED28. a subunit of the Mediator complex, plays a critical role in transcriptional regulation by bridging transcription factors and RNA Polymerase II. This evolutionarily conserved multiprotein complex is essential for modulating gene expression across eukaryotes. MED28 is part of the Mediator’s regulatory "kinase module," though its precise functional interactions remain under investigation. Studies suggest its involvement in cellular processes like proliferation, differentiation, and stress responses, with dysregulation linked to cancers and cardiovascular diseases.

MED28 antibodies are vital tools for exploring its molecular mechanisms and expression patterns. They enable detection and localization of MED28 in various experimental approaches, including Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Researchers utilize these antibodies to study MED28’s role in disease contexts, particularly its potential as a biomarker or therapeutic target. Commercial MED28 antibodies are typically raised against specific epitopes (e.g., human MED28 N-terminal regions) and validated for cross-reactivity in model organisms. However, batch variability and validation rigor remain considerations for experimental reproducibility. Ongoing research aims to clarify MED28’s tissue-specific functions and its interplay with other Mediator subunits in transcriptional programs.