The NDUFS1 antibody is a crucial tool for studying the NDUFS1 protein, a core subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) in the electron transport chain. NDUFS1 plays a pivotal role in NADH oxidation and proton translocation, essential for ATP production. Mutations in the NDUFS1 gene are linked to mitochondrial disorders, such as Leigh syndrome, and neurodegenerative diseases like Alzheimer’s and Parkinson’s. The antibody is widely used in research to detect NDUFS1 expression levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in the investigation of mitochondrial dysfunction. It also helps assess Complex I assembly and activity in diseases, cancer metabolism studies, and therapeutic response evaluations. Commercial NDUFS1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes with validation in knockout models. Its application extends to elucidating oxidative stress mechanisms and metabolic reprogramming in pathologies, making it vital for advancing mitochondrial biology and translational research.