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     Gel: 10+12%SDS-PAGE, Lysate: 40 μg, Lane: Human liver cancer tissue, Primary antibody: P00090(COX5B Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second    

  
  

The COX5B antibody is a crucial tool in biomedical research for studying cytochrome c oxidase (COX), also known as Complex IV, a key enzyme in the mitochondrial electron transport chain. COX catalyzes the final step of oxidative phosphorylation, transferring electrons to oxygen to form water while driving ATP synthesis. The COX enzyme comprises multiple subunits, with COX5B being a nuclear-encoded subunit critical for stabilizing the complex's structure and function.

COX5B antibodies are specifically designed to detect and quantify the COX5B protein in various experimental settings, such as Western blotting, immunohistochemistry, and immunofluorescence. These antibodies enable researchers to investigate COX5B expression levels, localization, and interactions in different tissues or disease models. Dysregulation of COX5B has been implicated in mitochondrial disorders, cancer, and neurodegenerative diseases, making its study vital for understanding metabolic dysfunction.

Commercial COX5B antibodies are typically raised in hosts like rabbits or mice using immunogenic peptide sequences unique to COX5B. Validation includes testing for specificity via knockout controls or siRNA knockdown. Researchers use these antibodies to explore mitochondrial bioenergetics, cellular responses to hypoxia, and metabolic adaptations in pathologies. Their applications extend to diagnostics and therapeutic development, particularly in diseases linked to oxidative phosphorylation defects.