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     Western blot analysis using RAD23B mouse mAb against K562 (1), Hela (2), A431 (3), HL-60 (4), Jurkat (5), A549 (6), HUVEC (7) and NIH/3T3 (8) cell lysate.    

  
  

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     Immunofluorescence analysis of Hela cells using RAD23B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Jurkat cells using RAD23B mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using RAD23B mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using RAD23B mouse mAb with DAB staining.    

  
  

The RAD23B antibody is a crucial tool for studying the RAD23 homolog B protein, which plays a dual role in nucleotide excision repair (NER) and the ubiquitin-proteasome system. RAD23B, along with its homolog RAD23A, contains ubiquitin-associated (UBA) and ubiquitin-like (UBL) domains, enabling interactions with proteasomes and damaged DNA-binding proteins. In NER, RAD23B partners with XPC to recognize and initiate repair of helix-distorting DNA lesions caused by UV radiation or chemicals. It stabilizes XPC and enhances its damage recognition efficiency. Additionally, RAD23B facilitates protein turnover by shuttling polyubiquitinated substrates to the proteasome for degradation.

Researchers use RAD23B antibodies primarily in Western blotting, immunoprecipitation, and immunofluorescence to investigate its expression, localization, and interactions in DNA repair mechanisms, cancer biology, and neurodegenerative diseases. Dysregulation of RAD23B is linked to carcinogenesis, as its overexpression in certain cancers may impair apoptosis, while reduced levels correlate with genomic instability. The antibody also aids in exploring RAD23B's role in modulating stress responses and regulating tumor suppressors like p53. Validated RAD23B antibodies are essential for distinguishing RAD23B from its homolog RAD23A and elucidating its tissue-specific functions, making it valuable for both basic research and clinical biomarker studies.