同用名/別名（Synonym）
Interleukin-3; IL-3; Hematopoietic Growth Factor; Mast Cell Growth Factor; MCGF; Multipotential Colony-Stimulating Factor; P-Cell-Stimulating Factor; IL3
描述（Description）
IL-3 protein is mainly secreted by T lymphocytes, mast cells and osteoblasts and is critical for the generation and differentiation of hematopoietic progenitor cells. It stimulates mature basophils, eosinophils and monocytes, promoting functional activation. IL-3 Protein, Human (His-Avi) is the recombinant human-derived IL-3 protein, expressed by E. coli , with N-His, N-Avi labeled tag. The total length of IL-3 Protein, Human (His-Avi) is 133 a.a., with molecular weight of ~16.9 kDa.
純度（Purity）
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
生物活性（Bioactivity）
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.02-0.1 ng/mL.
氨基酸序列（Target Protein Sequence）
APMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF
制劑（Trait）
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.
復(fù)溶（Reconstitution）
It is recommended to redissolve in sterile deionized water.
保存條件&效期（Storage & Stability）
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
運輸方式(Shipping)
Room temperature in continental US; may vary elsewhere.
生物活性（Bioactivity） | 
Determined by its ability to inhibit the proliferation of mouse MPC-11 cells.The expected ED50 is≤ 2.0 ng/ml, corresponding to a specific bioactivity of≥5 x 105 units/mg.
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電泳（SDS-PAGE） | 
Recombinant Human IL-3 SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y02101 SDS-PAGE. |
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關(guān)鍵字： GMP級;高純度;批間一致;生物活性;科研專供;
在生物科技領(lǐng)域，豐度蛋白專注重組蛋白供應(yīng)，以“豐度的風(fēng)帆為蛋白提供動力，豐度為蛋白護航”為使命，賦能生命科學(xué)發(fā)展。
蘇州豐度依托先進技術(shù)平臺，打造成熟生產(chǎn)體系，嚴(yán)控基因工程設(shè)計、細(xì)胞培養(yǎng)、蛋白純化等環(huán)節(jié)，優(yōu)化工藝與管理，確保產(chǎn)品高純度、高活性且批間一致。產(chǎn)品覆蓋基礎(chǔ)研究、生物醫(yī)藥研發(fā)等多領(lǐng)域，為科研及生產(chǎn)提供多樣、適配產(chǎn)品，助力突破技術(shù)難題 。