同用名/別名（Synonym）

Hepatocyte growth factor; HPTA; HGF; SF; Scatter factor; Hepatopoietin-A

描述（Description）

HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases and contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto oncogenic c-Met receptor. HGF is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.

純度（Purity）

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

生物活性（Bioactivity）

Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific bioactivity of ≥ 5 x 10⁵units/mg

氨基酸序列（Target Protein Sequence）

QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMG/VAIL NLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLR&VVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMG/VAIL NEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

制劑（Trait）

Lyophilized from a 0.22 μm filtered solution of PBS or PBS, pH 7.4, 8% trehalose 

復(fù)溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

保存條件&效期（Storage & Stability）

36 months at 2°C to 8°C in lyophilized state.

6 months at -20°C to -80°C under sterile conditions after reconstitution.

7-10 days at 2°C to 8°C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

運(yùn)輸方式(Shipping)

The product is shipped with blue ice.

生物活性（Bioactivity）

Measured by its ability to induce IL-11 secretion by Saos2 human osteosarcoma cells.

The ED50 for this effect is ≤2.5 ng/ml.

The bioactivity of Y01701 was higher than the other competing product.

電泳（SDS-PAGE）

Greater than 95% as determined by reducing SDS-PAGE. Human HGF is initially produced as a single, inactive polypeptide and is subsequently cleaved by serine proteases to generate a 69-kDa α chain and a 34-kDa β chain. The free α and β chains then combine to form an active heterodimer through the formation of disulfide bonds between cysteine residues. Under reducing conditions (SDS-PAGE), the protein exhibits apparent molecular masses of 90, 60, and 34 kDa, which correspond to the heavy α chain and the light β chain, respectively, due to glycosylation.

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