中文名稱:ROCK2抗體 | 英文名稱:Rabbit Polyclonal ROCK2 Antibody |
品牌: 艾普蒂 | 產(chǎn)地: 武漢 |
保存條件: Stored at -20°C for 3622 year. Avoid repeated freeze / thaw cycles. | 產(chǎn)品類別: 抗體 |
重組: 否 | 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA |
種屬反應(yīng)性: Human,Mouse,Rat | 宿主: Rabbit |
偶聯(lián)物: 無(wú) | 靶點(diǎn): ROCK2 |
WB | 1/500-1/2000 | Human,Mouse,Rat |
IF | 咨詢技術(shù) | Human,Mouse,Rat |
IHC | 1/50-1/200 | Human,Mouse,Rat |
ICC | 技術(shù)咨詢 | Human,Mouse,Rat |
FCM | 咨詢技術(shù) | Human,Mouse,Rat |
Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
Aliases | ROCK-II |
WB Predicted band size | 161 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | Fusion protein of human ROCK2 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse brain tissue lysate, Primary antibody: P07582(ROCK2 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes
The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P07582(ROCK2 Antibody) at dilution 1/130. (Original magnification: ×200)
以下是關(guān)于ROCK2抗體的3篇示例參考文獻(xiàn)(注:文獻(xiàn)信息為模擬示例,僅供參考):
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1. **文獻(xiàn)名稱**:*"ROCK2-Specific Antibody Reveals Its Role in Cancer Cell Invasion via Cytoskeletal Remodeling"*
**作者**:Li, X. et al.
**摘要**:本研究利用ROCK2特異性抗體,通過(guò)免疫印跡和免疫熒光技術(shù),揭示了ROCK2在乳腺癌細(xì)胞中通過(guò)調(diào)節(jié)肌動(dòng)蛋白骨架促進(jìn)侵襲轉(zhuǎn)移的機(jī)制,并證明其與患者預(yù)后不良相關(guān)。
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2. **文獻(xiàn)名稱**:*"Targeting ROCK2 with a Monoclonal Antibody Attenuates Neuroinflammation in Alzheimer's Disease Models"*
**作者**:Smith, J. & Patel, R.
**摘要**:文章報(bào)道了一種新型抗ROCK2單克隆抗體的開發(fā),并在阿爾茨海默病小鼠模型中驗(yàn)證其效果。結(jié)果顯示,該抗體通過(guò)抑制ROCK2活性減少tau蛋白過(guò)度磷酸化及神經(jīng)炎癥反應(yīng)。
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3. **文獻(xiàn)名稱**:*"ROCK2 Inhibition by a Neutralizing Antibody Improves Cardiac Fibrosis in Hypertensive Rats"*
**作者**:Yamamoto, K. et al.
**摘要**:研究使用ROCK2中和抗體干預(yù)高血壓模型大鼠,發(fā)現(xiàn)其能顯著降低心肌纖維化標(biāo)志物(如膠原沉積),提示ROCK2抗體在心血管疾病治療中的潛在應(yīng)用價(jià)值。
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如需真實(shí)文獻(xiàn),建議通過(guò)PubMed或Google Scholar搜索關(guān)鍵詞(如“ROCK2 antibody”、“ROCK2 inhibitor”),并篩選近年高影響力期刊的研究(如*Nature Cell Biology*、*Journal of Biological Chemistry*等)。
The Rho-associated coiled-coil kinase 2 (ROCK2) antibody is a crucial tool for studying the ROCK2 protein, a serine/threonine kinase belonging to the AGC family of kinases. ROCK2. along with its isoform ROCK1. plays a pivotal role in regulating cytoskeletal dynamics, cell adhesion, migration, and apoptosis through its interaction with Rho GTPases, particularly RhoA. It mediates downstream signaling by phosphorylating substrates such as myosin light chain (MLC) and LIM kinase, influencing actomyosin contractility and stress fiber formation.
ROCK2 is implicated in diverse physiological and pathological processes, including cardiovascular remodeling, neuronal degeneration, cancer metastasis, and fibrosis. Its overexpression or hyperactivity has been linked to hypertension, atherosclerosis, and glaucoma. The ROCK2 antibody enables detection and quantification of ROCK2 expression in tissues and cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. It also aids in elucidating ROCK2's spatial-temporal localization and activation status under experimental or disease conditions.
Researchers frequently use ROCK2-specific antibodies to validate gene silencing (e.g., siRNA/CRISPR) or assess pharmacological inhibition by compounds like fasudil or Y-27632. As ROCK2-selective inhibitors gain traction in drug development, high-specificity antibodies are essential for distinguishing ROCK2 from ROCK1. which share ~65% homology but exhibit distinct tissue distributions and functions. This underscores the antibody's importance in both basic research and therapeutic targeting of ROCK2-associated pathways.
成立日期 | 2024-06-24 (2年) | 注冊(cè)資本 | 50萬(wàn)(元) |
員工人數(shù) | 1-10人 | 年?duì)I業(yè)額 | ¥ 100萬(wàn)以內(nèi) |
主營(yíng)行業(yè) | 中間體,化學(xué)試劑 | 經(jīng)營(yíng)模式 | 貿(mào)易,工廠,試劑,定制,服務(wù) |
產(chǎn)品名稱 | 價(jià)格 | 公司名稱 | 報(bào)價(jià)日期 | |
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¥1098 |
VIP3年
|
上海滬震實(shí)業(yè)有限公司
|
2025-08-11 | |
詢價(jià) |
VIP5年
|
上海冠導(dǎo)生物工程有限公司
|
2025-08-11 | |
¥1600.00 |
VIP10年
|
北京索萊寶科技有限公司
|
2025-08-11 |