resDetect? Human FLT3L ELISA Kit (Residue Testing)

背景（Background）

Human FLT3L ELISA Kit is based on ELISA sandwich method and designed to measure human FLT3L levels in cell culture supernates, serum, and plasma. It contains recombinant human FLT3L and a pair of antibodies against the recombinant human FLT3L, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of GMP human FLT3L (ACROBiosystems, cat#GMP-FLLH28) concentrations. The specificity has been verified.

應(yīng)用說明（Application）

The kit is developed for the detection and quantitative determination of GMP human FLT3L in human serum and cell culture supernates.

It is for research use only.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存儲(chǔ)（Storage）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human FLT3L. The kit consists of Pre-coated Anti-FLT3L Antibody Microplate and Human FLT3L Standard and Biotin-Anti-FLT3L Antibody and Streptavidin-HRP and buffers.

Your experiment will include 6 simple steps:

a) Bring all reagents to room temperature(20℃-25℃) before use.

b) Add your sample to the plate and take the Human FLT3L as standard. The samples and standard are diluted by Dilution Buffer.

c) Wash the plate and add the Biotin-Anti-FLT3L Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

e) Wash the plate and add TMB.

f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.

背景（Background）：FLT3L

FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.