Human Kremen-2 protein, His Tag

分子別名（Synonym）

KREMEN2, Kremen-2, KRM2, Dickkopf receptor 2,Kringle domain-containing transmembrane protein 2

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Kremen-2, His Tag (KR2-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Ala 364 (Accession # Q8NCW0-1).

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 38.0 kDa. The protein migrates as 45-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.2M Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Kremen2（Krm2）作為經(jīng)典Wnt/β-catenin信號通路的關(guān)鍵調(diào)節(jié)因子，在胚胎發(fā)育、骨形成和腫瘤發(fā)生中起著重要作用。Krm1及其同源Krm2具有結(jié)合Dkk1和抑制Wnt信號傳導(dǎo)的能力，這兩個(gè)過程之前都被證明依賴于細(xì)胞外結(jié)構(gòu)域。先前的研究表明，Krm2可能參與胚胎發(fā)育、骨形成、神經(jīng)脊形成和腫瘤發(fā)生，并可能是癌癥分級的生物標(biāo)志物和潛在的治療靶點(diǎn)。