Mouse Dkk-1 Protein, His Tag

分子別名（Synonym）

DKK1,SK

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse Dkk-1, His Tag (DK1-M52H6) is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 272 (Accession # O54908).

Predicted N-terminus: Thr 32

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 28.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

dickkopf相關(guān)蛋白家族（DKK-1、-2、-3和-4）的成員是分泌蛋白，具有兩個(gè)富含半胱氨酸的結(jié)構(gòu)域，由接頭區(qū)隔開(kāi)。DKK1通過(guò)抑制WNT信號(hào)通路參與胚胎發(fā)育，以高親和力與LRP6結(jié)合，并阻止Frizzled-WNT-LRP6復(fù)合物對(duì)Wnts的反應(yīng)形成。DKK1與細(xì)胞表面受體Kremen形成三元復(fù)合物時(shí)，促進(jìn)LRP6內(nèi)化和降解。DKK1在發(fā)育過(guò)程中不僅起頭部誘導(dǎo)劑的作用，還調(diào)節(jié)關(guān)節(jié)重塑和骨形成，這表明DKK1在類(lèi)風(fēng)濕性關(guān)節(jié)炎和多發(fā)性骨髓瘤的發(fā)病機(jī)制中起著重要作用。最近的研究報(bào)告稱(chēng)，DKK1從一個(gè)確定的發(fā)育時(shí)間點(diǎn)開(kāi)始影響眼睛發(fā)育，并且通過(guò)β-catenin介導(dǎo)的Pdgfrα和E-cadherin表達(dá)對(duì)晶狀體與表面外胚層的分離至關(guān)重要。