Human CTGF / CCN2 Protein, His Tag

分子別名（Synonym）

CCN2,NOV2,HCS24,IGFBP8,IBP-8,IGFBP-8,IGF-binding protein 8

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human CTGF, His Tag (CTF-H52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ala 349 (Accession # Q5M8T4-1).

Predicted N-terminus: Gln 27

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 37.4 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Potassium glutamate and Arginine, pH7.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

結(jié)締組織生長(zhǎng)因子（CTGF），也稱(chēng)為CCN2，是模塊化基質(zhì)細(xì)胞蛋白CCN（CCN1-6）家族的成員。與其他CCN蛋白一樣，成熟的人CTGF由IGF結(jié)合蛋白結(jié)構(gòu)域、vWF-C結(jié)構(gòu)域、TSP-1結(jié)構(gòu)域和半胱氨酸結(jié)肝素結(jié)合結(jié)構(gòu)域組成。CTGF促進(jìn)軟骨細(xì)胞的增殖和分化。在許多細(xì)胞類(lèi)型中介導(dǎo)肝素和二價(jià)陽(yáng)離子依賴(lài)性細(xì)胞粘附，包括成纖維細(xì)胞、肌成纖維細(xì)胞，內(nèi)皮細(xì)胞和上皮細(xì)胞。增強(qiáng)成纖維細(xì)胞生長(zhǎng)因子誘導(dǎo)的DNA合成。CCN2在體內(nèi)的功能分析主要集中在其作為多種纖維化疾病中過(guò)量ECM合成的介質(zhì)的關(guān)鍵作用上。