CHAPS 是一種用于膜生物化學(xué)的非變性兩性離子洗滌劑。

可用于溶解膜蛋白以及打斷蛋白-蛋白相互作用。CHAPS 具有低膠束分子量 (6,150) 和高臨界膠束濃度 (6-10mM)，因此可通過(guò)透析從樣品中除去。它也適合在等電聚焦和二維電泳中用于溶解蛋白質(zhì)。CHAPS 常用于非變性（無(wú)尿素）等電聚焦，已發(fā)現(xiàn)它對(duì)某些亞細(xì)胞制備物和植物蛋白能夠得出優(yōu)異的分辨率。通常將介于 2-4% (w/v) 間的濃度用于等電聚焦凝膠。

CHAPS is a non-denaturing, zwitterionic detergent derived from cholic acid. CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions and is electrically neutral. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. The critical micelle concentration of CHAPS is 6-10mM.
A non-denaturing, zwitterionic detergent for membrane proteins

Application：

Buffer; zwitterionic nondenaturing detergent for solubilizing membrane proteins CHAPS is used in the laboratory to dissolve biological macromolecules such as proteins. It acts as a non-denaturing solvent used in purification of membrane proteins. It is also useful in conjunction with nonionic detergents like Triton X-100. Further, it is used as a non-denaturing zwitterionic detergent for membrane biochemistry.