癌胚抗原相關細胞粘附分子7(CEACAM7)重組蛋白
[ PROPERTIES ]
Residues: Lys147~Thr231
Tags: N-terminal His-Tag
Accession: Q14002
Host: E. coli
Subcellular Location: Cell membrane;
Lipid-anchor, GPI-anchor. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.0
Predicted Molecular Mass: 11.0kDa
Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducingconditions.Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget
protein. The loss rate was determined by accelerated thermal degradationtest,
that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
KPSI TSNNFNPVEN KDIVVLTCQP ETQNTTYLWW VNNQSLLVSP RLLLSTDNRTLVLLSATKND IGPYECEIQN PVGASRSDPV T
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關鍵字： CGM2;CEA;CEACAM7;癌胚抗原相關細胞粘附分子7(CEACAM;
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上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠大； 更是一家專注于生命科學與生物技術領域，集產品研發(fā)、生產、銷售為一體的高科技公司，并立志以競爭力的價格向客戶輸送高質量的生物試劑。主要研究領域是生命科學，包括分子生物學、細胞生物學、免疫學試劑、重組蛋白，抗體，實驗技術服務等多個應用領域。主要產品有：Elisa檢測試劑盒、PCR檢測試劑盒、抗體、重組蛋白，蛋白定制，細胞系，原代細胞，常用實驗試劑，染色液等科研產品。