X-GAL NEW
| Price | $30 | $59 |
| Package | 100mg | 500mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-23 |
Product Details
| Product Name: X-GAL | CAS No.: 7240-90-6 |
| Purity: 99.65% | Supply Ability: 10g |
| Release date: 2025/07/23 |
Product Introduction
Bioactivity
| Name | X-GAL |
| Description | X-GAL (BCIG) is a widely used chromogenic substrate for β-galactosidase, which cleaves X-GAL to produce an insoluble blue compound. |
| In vitro | Instructions: I. Solution preparation Mother solution preparation: Prepare X-GAL stock solution: Dissolve X-GAL in anhydrous dimethylformamide (DMF) or dimethyl sulfoxide (DMSO) to prepare a 20 mg/mL stock solution. Note: Protect from light, dispense in small volumes (e.g. 50–100 μL), and store at -20°C. The storage time should not be too long to avoid degradation. II. Operation steps 1. Prepare color reaction mixture 1) For plate screening: Add X-GAL and IPTG (isopropyl-β-D-thiogalactoside) to LB agar plates. Common final concentrations: X-GAL 40 μg/mL, IPTG 0.1 mM. Add X-GAL and IPTG when the agar medium cools to about 50°C, mix well, and pour onto the plate. 2). For liquid color development: Add X-GAL and IPTG to the reaction buffer, the usual concentration is the same as for plate screening. 2. Experimental application Blue-white screening: 1) Transform the vector containing the reporter gene (such as lacZ or lacZα) into the host bacteria (such as E. coli DH5α). 2) Spread on a plate containing X-GAL and IPTG and culture at 37°C for 12–16 hours. 3) Screening results: Blue colonies indicate positive β-gal activity (lacZ expression), and white colonies indicate negative (lacZ is not expressed). Tissue staining: 1) After appropriate tissue fixation and permeabilization, use a reaction buffer containing X-GAL (usually PBS at pH 7.2–7.5, containing Mg2?). 2) Incubate at 37°C for 12–24 hours, and observe the blue precipitate under a microscope for color development. |
| Storage | keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO : 40 mg/mL (97.89 mM), Sonication is recommended. |
| Keywords | X-GAL | XGAL | X GAL | Inhibitor | inhibit |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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