Rhodamine 123 NEW
| Price | $47 | $61 | $82 |
| Package | 5mg | 10mg | 25mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-07-16 |
Product Details
| Product Name: Rhodamine 123 | CAS No.: 62669-70-9 |
| Purity: 99.23% | Supply Ability: 10g |
| Release date: 2025/07/16 |
Product Introduction
Bioactivity
| Name | Rhodamine 123 |
| Description | Rhodamine 123 is a fluorescent dye (λex=503 nm, λem=527 nm). |
| Cell Research | Instructions I. Solution preparation 1. Preparation of mother solution: Dissolve Rhodamine 123 in anhydrous DMSO or ethanol to prepare a 1-5 mM stock solution. ? Note: Avoid vigorous stirring or shaking during dissolution to prevent fluorescence quenching. 2. Working solution preparation: Dilute the stock solution with appropriate culture medium to the required working concentration, usually 1-2 μM. II. Operation steps 1. Cell preparation: 1) Culture cells on a slide, the cell density should be 5×10^4 to 5×10^5 cells/mL. ? 2) Wash cells with PBS or Hank’s solution to remove residual culture medium. 2. Staining: Add the working concentration solution to the cells and incubate at 37°C in the dark for 30 minutes to 1 hour. ? 3. Washing: 1) Remove the dye solution and wash the cells with culture medium or PBS to remove unbound dye. 2) If cells need to be fixed, add 10% formalin buffer and incubate for 15-20 minutes, then wash with PBS. ? 4. Observation: Use a fluorescence microscope with a fluorescein filter to observe cells. Rhodamine 123 is excited at a wavelength of about 507 nm and emits green fluorescence at 529 nm. ? Precautions: 1) Wear gloves during operation to avoid contact between skin or mucous membranes and reagents. ? 2) Avoid light during incubation and storage to prevent fluorescence quenching. ? 3) After staining, fluorescence detection analysis should be performed immediately. |
| In vitro | The intensity of R123 fluorescence has a peak at a concentration of 50 μM and decreases to zero at higher concentrations because of self-quenching[1]. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 30 mg/mL (78.78 mM), Sonication is recommended. |
| Keywords | Rhodamine-123 | Rhodamine123 | Rhodamine 123 | RH123 | RH 123 | R22420 | R 22420 | Inhibitor | inhibit |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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